A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers
Ischemic heart disease (IHD) is the most common cause of cardiovascular mortality in Malaysia and around the globe. New novel biomarker, Peroxisome Proliferator-Activated Receptors-Alpha (PPAR-a) was investigated in this study to give significant contribution to early detection of IHD. In this st...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2008
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/48977/ http://eprints.usm.my/48977/1/FONG%20SIEW%20WAI%20-%2024%20pages.pdf |
| _version_ | 1848881309076684800 |
|---|---|
| author | Fong Slew, Wai |
| author_facet | Fong Slew, Wai |
| author_sort | Fong Slew, Wai |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Ischemic heart disease (IHD) is the most common cause of cardiovascular
mortality in Malaysia and around the globe. New novel biomarker, Peroxisome
Proliferator-Activated Receptors-Alpha (PPAR-a) was investigated in this study
to give significant contribution to early detection of IHD. In this study, we
investigated the mRNA expression and protein levels of both PPAR-a and
C-Reactive Protein (CRP) genes in the peripheral blood of IHD patients (n=10)
and apparently healthy volunteers (n=1 0). The mRNA levels of PPAR-a (native
and truncated) and CRP were determined by reverse transcription-polymerase
chain reaction. The protein expression was evaluated by Western blot and
measured by Enzyme linked immunosorbent assay (ELISA). The CRP mRNA
expression and serum levels increased significantly (p<0.05) in IHD patients
compared to normal volunteers. There was presence of both native active and
truncated inhibitory PPAR-a protein in human blood leukocytes. The mRNA
expressions of both native and truncated variants of PPAR-a were lower (p
<0.05) in IHD patients group than those in the normal volunteers group.
However, there was no significant difference (p>0.05) in PPAR-a protein level
between IHD patients and normal volunteers. These findings suggest that
mRNA expression level of both PPAR-a variants is decreased in IHD patients.
An over-expression of the truncated inhibitory isoform of PPAR-a protein may be
involved in the pathogenesis of IHD. Further study will be conducted to elucidate
this matter. |
| first_indexed | 2025-11-15T18:16:58Z |
| format | Monograph |
| id | usm-48977 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:16:58Z |
| publishDate | 2008 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-489772021-04-21T08:00:57Z http://eprints.usm.my/48977/ A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers Fong Slew, Wai R Medicine (General) Ischemic heart disease (IHD) is the most common cause of cardiovascular mortality in Malaysia and around the globe. New novel biomarker, Peroxisome Proliferator-Activated Receptors-Alpha (PPAR-a) was investigated in this study to give significant contribution to early detection of IHD. In this study, we investigated the mRNA expression and protein levels of both PPAR-a and C-Reactive Protein (CRP) genes in the peripheral blood of IHD patients (n=10) and apparently healthy volunteers (n=1 0). The mRNA levels of PPAR-a (native and truncated) and CRP were determined by reverse transcription-polymerase chain reaction. The protein expression was evaluated by Western blot and measured by Enzyme linked immunosorbent assay (ELISA). The CRP mRNA expression and serum levels increased significantly (p<0.05) in IHD patients compared to normal volunteers. There was presence of both native active and truncated inhibitory PPAR-a protein in human blood leukocytes. The mRNA expressions of both native and truncated variants of PPAR-a were lower (p <0.05) in IHD patients group than those in the normal volunteers group. However, there was no significant difference (p>0.05) in PPAR-a protein level between IHD patients and normal volunteers. These findings suggest that mRNA expression level of both PPAR-a variants is decreased in IHD patients. An over-expression of the truncated inhibitory isoform of PPAR-a protein may be involved in the pathogenesis of IHD. Further study will be conducted to elucidate this matter. Universiti Sains Malaysia 2008-10 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48977/1/FONG%20SIEW%20WAI%20-%2024%20pages.pdf Fong Slew, Wai (2008) A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Fong Slew, Wai A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title | A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title_full | A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title_fullStr | A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title_full_unstemmed | A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title_short | A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers |
| title_sort | study of peroxisome proliferator-activated receptor (ppar) alpha in patients with ischemic heart disease and heal thy volunteers |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/48977/ http://eprints.usm.my/48977/1/FONG%20SIEW%20WAI%20-%2024%20pages.pdf |