Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor
Cancer generally is a genetic disease that can cause alteration in specific gene in which a group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily and involved in many cellular fun...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2008
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| Online Access: | http://eprints.usm.my/48854/ http://eprints.usm.my/48854/1/MUHAMMAD%20REDZWAN%20BIN%20SIDIK%20-%2024%20%20pages.pdf |
| _version_ | 1848881274533445632 |
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| author | Muhammad Redzwan, Sidik |
| author_facet | Muhammad Redzwan, Sidik |
| author_sort | Muhammad Redzwan, Sidik |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Cancer generally is a genetic disease that can cause alteration in specific gene in which a
group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box
binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily
and involved in many cellular functions, including transcriptional regulation, translational
regulation, DNA repair, drug resistance and stress responses to extracellular signals. YB-1
gene can be found in a cytoplasm and nuclear cell. The YB-1 gene comprises 8 exons
spanning 19 kb of genomic DNA and is located on chromosome I p34. The aim of the
study is to differential relatively quantitative the expression of YB-I gene in brain tumor
sample (meningioma) and normal brain sample (Astrocyte SVG I2). SYBR-Green I
based Real-Time PCR was performed and data analysis using formula 2-MCt has
demonstrated that YB-1 gene was over-expressed by 2.86 fold in comparison to its
expression in normal sample. This preliminary data is very important in the future
experiment for expression of YB-I gene showed the potential as a biology marker for
detection of brain tumor. |
| first_indexed | 2025-11-15T18:16:25Z |
| format | Monograph |
| id | usm-48854 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:16:25Z |
| publishDate | 2008 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-488542021-04-13T01:47:18Z http://eprints.usm.my/48854/ Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor Muhammad Redzwan, Sidik R Medicine (General) Cancer generally is a genetic disease that can cause alteration in specific gene in which a group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily and involved in many cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug resistance and stress responses to extracellular signals. YB-1 gene can be found in a cytoplasm and nuclear cell. The YB-1 gene comprises 8 exons spanning 19 kb of genomic DNA and is located on chromosome I p34. The aim of the study is to differential relatively quantitative the expression of YB-I gene in brain tumor sample (meningioma) and normal brain sample (Astrocyte SVG I2). SYBR-Green I based Real-Time PCR was performed and data analysis using formula 2-MCt has demonstrated that YB-1 gene was over-expressed by 2.86 fold in comparison to its expression in normal sample. This preliminary data is very important in the future experiment for expression of YB-I gene showed the potential as a biology marker for detection of brain tumor. Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48854/1/MUHAMMAD%20REDZWAN%20BIN%20SIDIK%20-%2024%20%20pages.pdf Muhammad Redzwan, Sidik (2008) Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Muhammad Redzwan, Sidik Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor |
| title | Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| title_full | Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| title_fullStr | Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| title_full_unstemmed | Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| title_short | Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| title_sort | investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/48854/ http://eprints.usm.my/48854/1/MUHAMMAD%20REDZWAN%20BIN%20SIDIK%20-%2024%20%20pages.pdf |