Antimicrobial properties of Euphorbia hirta crude extract

Antimicrobial effects from the extracted whole plant of Euphorbia hirta were studied against several Gram positive and Gram negative bacteria. Water and petroleum ether fraction of the extract have been isolated by using the soxhet extraction method. The Minimum Inhibitory Concentration (MIC) tes...

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Main Author: Jusoh, Ahmad Fadly
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2006
Subjects:
Online Access:http://eprints.usm.my/48709/
http://eprints.usm.my/48709/1/AHMAD%20FADLY%20JUSOH-24%20pages.pdf
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author Jusoh, Ahmad Fadly
author_facet Jusoh, Ahmad Fadly
author_sort Jusoh, Ahmad Fadly
building USM Institutional Repository
collection Online Access
description Antimicrobial effects from the extracted whole plant of Euphorbia hirta were studied against several Gram positive and Gram negative bacteria. Water and petroleum ether fraction of the extract have been isolated by using the soxhet extraction method. The Minimum Inhibitory Concentration (MIC) tests were done to determine the minimum concentration of the crude extract that can inhibit bacterial growth. The minimum inhibitory concentration of both extract are 25.00 mg/ml (water extract) and 0.025 mg/ml (petroleum ether extract) respectively. Agar diffusion test was also done by impregnating different concentration of the water extract- 100 mg/ml, 50 mg/ml, 25 mg/ml and the petroleum ether extract- 0.1 mg/ml, 0.05 mg/ml, 0.025 mg/ml respectively onto the standard blank disc. Significant inhibition zone were observed with 0.1 mg/ml of petroleum ether fraction against methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis on a nutrient agar plates. However, no inhibition zones were observed against the Gram negative bacterial plates consisted of Escherichia coli (ATCC 25922) and Salmonella sp. for both extracts. This result however, primarily validates that the petroleum ether Euphorbia hirta crude extract has shown to be a promising effective antibacterial agents against certain Gram positive bacteria.
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language English
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spelling usm-487092021-03-29T01:53:43Z http://eprints.usm.my/48709/ Antimicrobial properties of Euphorbia hirta crude extract Jusoh, Ahmad Fadly R Medicine Antimicrobial effects from the extracted whole plant of Euphorbia hirta were studied against several Gram positive and Gram negative bacteria. Water and petroleum ether fraction of the extract have been isolated by using the soxhet extraction method. The Minimum Inhibitory Concentration (MIC) tests were done to determine the minimum concentration of the crude extract that can inhibit bacterial growth. The minimum inhibitory concentration of both extract are 25.00 mg/ml (water extract) and 0.025 mg/ml (petroleum ether extract) respectively. Agar diffusion test was also done by impregnating different concentration of the water extract- 100 mg/ml, 50 mg/ml, 25 mg/ml and the petroleum ether extract- 0.1 mg/ml, 0.05 mg/ml, 0.025 mg/ml respectively onto the standard blank disc. Significant inhibition zone were observed with 0.1 mg/ml of petroleum ether fraction against methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis on a nutrient agar plates. However, no inhibition zones were observed against the Gram negative bacterial plates consisted of Escherichia coli (ATCC 25922) and Salmonella sp. for both extracts. This result however, primarily validates that the petroleum ether Euphorbia hirta crude extract has shown to be a promising effective antibacterial agents against certain Gram positive bacteria. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2006-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48709/1/AHMAD%20FADLY%20JUSOH-24%20pages.pdf Jusoh, Ahmad Fadly (2006) Antimicrobial properties of Euphorbia hirta crude extract. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
spellingShingle R Medicine
Jusoh, Ahmad Fadly
Antimicrobial properties of Euphorbia hirta crude extract
title Antimicrobial properties of Euphorbia hirta crude extract
title_full Antimicrobial properties of Euphorbia hirta crude extract
title_fullStr Antimicrobial properties of Euphorbia hirta crude extract
title_full_unstemmed Antimicrobial properties of Euphorbia hirta crude extract
title_short Antimicrobial properties of Euphorbia hirta crude extract
title_sort antimicrobial properties of euphorbia hirta crude extract
topic R Medicine
url http://eprints.usm.my/48709/
http://eprints.usm.my/48709/1/AHMAD%20FADLY%20JUSOH-24%20pages.pdf