Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia

The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Mitochondrial DNA (mtDNA) haplogroups were identified on the basis of the presence or absence of a restriction enzyme polymorphism in the coding region along with the...

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Main Author: Mokhtaruddin, Nor Alfarizan
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2006
Subjects:
Online Access:http://eprints.usm.my/48500/
http://eprints.usm.my/48500/1/Nor%20Alfarizan%20Binti%20Mokhtaruddin.pdf
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author Mokhtaruddin, Nor Alfarizan
author_facet Mokhtaruddin, Nor Alfarizan
author_sort Mokhtaruddin, Nor Alfarizan
building USM Institutional Repository
collection Online Access
description The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Mitochondrial DNA (mtDNA) haplogroups were identified on the basis of the presence or absence of a restriction enzyme polymorphism in the coding region along with the sequence polymorphisms in the control region. This study was undertaken to analyze the mtDNA polymorphism in random population of Chinese ethnic of Peninsular Malaysia. A total number of 44 buccal cells samples were collected and subjected to extraction, followed by amplification by PCR and digestion process through restriction fragment length polymorphism (RFLP) analysis. Alu I restriction enzyme was used to detect polymorphism at nucleotide position 10397 in the mtDNA coding regions. Result shows that out of 44 samples, 21 of them were identified to be having an Alu I site gain at nucleotide position 10397, which classified them under the superhaplogroup M.
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institution Universiti Sains Malaysia
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language English
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publishDate 2006
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spelling usm-485002021-03-09T04:18:01Z http://eprints.usm.my/48500/ Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia Mokhtaruddin, Nor Alfarizan R Medicine (General) The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Mitochondrial DNA (mtDNA) haplogroups were identified on the basis of the presence or absence of a restriction enzyme polymorphism in the coding region along with the sequence polymorphisms in the control region. This study was undertaken to analyze the mtDNA polymorphism in random population of Chinese ethnic of Peninsular Malaysia. A total number of 44 buccal cells samples were collected and subjected to extraction, followed by amplification by PCR and digestion process through restriction fragment length polymorphism (RFLP) analysis. Alu I restriction enzyme was used to detect polymorphism at nucleotide position 10397 in the mtDNA coding regions. Result shows that out of 44 samples, 21 of them were identified to be having an Alu I site gain at nucleotide position 10397, which classified them under the superhaplogroup M. Universiti Sains Malaysia 2006 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48500/1/Nor%20Alfarizan%20Binti%20Mokhtaruddin.pdf Mokhtaruddin, Nor Alfarizan (2006) Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia. Other. Universiti Sains Malaysia. (Submitted)
spellingShingle R Medicine (General)
Mokhtaruddin, Nor Alfarizan
Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title_full Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title_fullStr Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title_full_unstemmed Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title_short Classification of Superbaplogroup M in Chinese Population of Peninsular Malaysia
title_sort classification of superbaplogroup m in chinese population of peninsular malaysia
topic R Medicine (General)
url http://eprints.usm.my/48500/
http://eprints.usm.my/48500/1/Nor%20Alfarizan%20Binti%20Mokhtaruddin.pdf