Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/48310/ http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf |
| _version_ | 1848881118715052032 |
|---|---|
| author | Chung, Wan Jie |
| author_facet | Chung, Wan Jie |
| author_sort | Chung, Wan Jie |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing. |
| first_indexed | 2025-11-15T18:13:56Z |
| format | Thesis |
| id | usm-48310 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:13:56Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-483102021-02-16T06:24:16Z http://eprints.usm.my/48310/ Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis Chung, Wan Jie R735-854 Medical education. Medical schools. Research Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing. 2018-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf Chung, Wan Jie (2018) Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Chung, Wan Jie Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title | Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_full | Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_fullStr | Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_full_unstemmed | Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_short | Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_sort | chromatographic enrichment of bioactive quassinoids in tongkat ali for the improvement of spermatogenesis |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/48310/ http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf |