Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegan...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/48166/ http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf |
| _version_ | 1848881079684956160 |
|---|---|
| author | Zain, Mariani Mohd |
| author_facet | Zain, Mariani Mohd |
| author_sort | Zain, Mariani Mohd |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group. |
| first_indexed | 2025-11-15T18:13:19Z |
| format | Thesis |
| id | usm-48166 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:13:19Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-481662021-01-22T01:03:27Z http://eprints.usm.my/48166/ Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans Zain, Mariani Mohd QH1 Natural history (General - Including nature conservation, geographical distribution) Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group. 2019-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf Zain, Mariani Mohd (2019) Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Zain, Mariani Mohd Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title | Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title_full | Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title_fullStr | Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title_full_unstemmed | Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title_short | Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans |
| title_sort | phenotypic and proteomic analysis associated with macrocyclic lactones-ivermectin exposure in caenorhabditis elegans |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/48166/ http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf |