Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the majo...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/48149/ http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf |
| _version_ | 1848881075271499776 |
|---|---|
| author | Al Kanan, Alaa Taha Yasir |
| author_facet | Al Kanan, Alaa Taha Yasir |
| author_sort | Al Kanan, Alaa Taha Yasir |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the major drawback effects of tamoxifen, particularly at 10-15 years of post-treatment. Thereafter, breast cancer recurrence may occur which also contribute to the major causes of breast cancer-related deaths. Garcinia hombroniana (GH) (seashore mangosteen) is a plant that has been reported to possess good cytotoxic effect against the growth of various human cancer cell lines, including the MCF-7. For this reason, the aim of this study was to further investigate the anti-proliferative and apoptotic effects of the essential oil extracted from the leaves of GH against two different types of breast cancer cell lines, MCF-7 and MCF-7/TAMR-1.
Methods: The essential oil was extracted by hydrodistillation process from the leaves of GH. The anti-proliferative effects of the essential oil against MCF-7 and MCF-7/TAMR-1 cancer cell lines were determined using MTT assay and measured by a microplate reader. The mechanism of cell death was determined using Annexin V-FITC/propidium iodide staining assay and quantitatively measured by flow cytometry. The human non-cancerous breast cell line, MCF-10A was also included in both assays as comparative control cells to the MCF-7 and MCF-7/TAMR-1 cells. |
| first_indexed | 2025-11-15T18:13:15Z |
| format | Thesis |
| id | usm-48149 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:13:15Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-481492021-01-20T07:58:29Z http://eprints.usm.my/48149/ Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines Al Kanan, Alaa Taha Yasir SB Plant culture SB610-615 Weeds, parasitic plants, etc. Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the major drawback effects of tamoxifen, particularly at 10-15 years of post-treatment. Thereafter, breast cancer recurrence may occur which also contribute to the major causes of breast cancer-related deaths. Garcinia hombroniana (GH) (seashore mangosteen) is a plant that has been reported to possess good cytotoxic effect against the growth of various human cancer cell lines, including the MCF-7. For this reason, the aim of this study was to further investigate the anti-proliferative and apoptotic effects of the essential oil extracted from the leaves of GH against two different types of breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Methods: The essential oil was extracted by hydrodistillation process from the leaves of GH. The anti-proliferative effects of the essential oil against MCF-7 and MCF-7/TAMR-1 cancer cell lines were determined using MTT assay and measured by a microplate reader. The mechanism of cell death was determined using Annexin V-FITC/propidium iodide staining assay and quantitatively measured by flow cytometry. The human non-cancerous breast cell line, MCF-10A was also included in both assays as comparative control cells to the MCF-7 and MCF-7/TAMR-1 cells. 2019 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf Al Kanan, Alaa Taha Yasir (2019) Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | SB Plant culture SB610-615 Weeds, parasitic plants, etc. Al Kanan, Alaa Taha Yasir Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title | Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title_full | Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title_fullStr | Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title_full_unstemmed | Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title_short | Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines |
| title_sort | mechanisms of anti-prolifertative effect of garcinia hombroniana essential oils leaves in mcf-7 and mcf-7/tamr-1 human breast cancer cell lines |
| topic | SB Plant culture SB610-615 Weeds, parasitic plants, etc. |
| url | http://eprints.usm.my/48149/ http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf |