The study of Interacting Partners of a Transcription Factor, YB-1
The transcription factor, YB-1 was considered to be a transcription factor. YB-1 also involved in many genes regulation, such as genes involved in cell proliferation and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate dependent-transcriptional enhancing, tiss...
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| Format: | Thesis |
| Language: | English |
| Published: |
2005
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| Online Access: | http://eprints.usm.my/47843/ http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf |
| _version_ | 1848880990801362944 |
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| author | Mansor, Mohamad Hafizi |
| author_facet | Mansor, Mohamad Hafizi |
| author_sort | Mansor, Mohamad Hafizi |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The transcription factor, YB-1 was considered to be a transcription factor. YB-1
also involved in many genes regulation, such as genes involved in cell proliferation
and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate
dependent-transcriptional enhancing, tissue cell specific processes, stress
response, immune response (major histocompatibility class II genes), and many more.
Because of several important of YB-1, my supervisor proposed to study of YB-
1 's interacting partners. Base on interacting partner of YB-1, we hopefully can explore
properties of YB-1.
In this study, we used culture ofHeLa cells, and lysis the cells using Single-step
lysis for extraction of total cellular protein, and nuclear and cytoplasmic
proteinextraction methods to get YB-1 protein. Using co-immunoprecipitation analysis
and several antibodies, we will detect interacting partners of YB-1.
Finally after co-immunoprecipitation analysis, we got YB-1 protein has
specific interaction with P-glycoprotein mdr 1 protein which involved in multidrugresistant
cancer cells. |
| first_indexed | 2025-11-15T18:11:54Z |
| format | Thesis |
| id | usm-47843 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:11:54Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-478432020-11-03T07:49:44Z http://eprints.usm.my/47843/ The study of Interacting Partners of a Transcription Factor, YB-1 Mansor, Mohamad Hafizi RC Internal medicine The transcription factor, YB-1 was considered to be a transcription factor. YB-1 also involved in many genes regulation, such as genes involved in cell proliferation and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate dependent-transcriptional enhancing, tissue cell specific processes, stress response, immune response (major histocompatibility class II genes), and many more. Because of several important of YB-1, my supervisor proposed to study of YB- 1 's interacting partners. Base on interacting partner of YB-1, we hopefully can explore properties of YB-1. In this study, we used culture ofHeLa cells, and lysis the cells using Single-step lysis for extraction of total cellular protein, and nuclear and cytoplasmic proteinextraction methods to get YB-1 protein. Using co-immunoprecipitation analysis and several antibodies, we will detect interacting partners of YB-1. Finally after co-immunoprecipitation analysis, we got YB-1 protein has specific interaction with P-glycoprotein mdr 1 protein which involved in multidrugresistant cancer cells. 2005 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf Mansor, Mohamad Hafizi (2005) The study of Interacting Partners of a Transcription Factor, YB-1. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | RC Internal medicine Mansor, Mohamad Hafizi The study of Interacting Partners of a Transcription Factor, YB-1 |
| title | The study of Interacting Partners of a
Transcription Factor, YB-1 |
| title_full | The study of Interacting Partners of a
Transcription Factor, YB-1 |
| title_fullStr | The study of Interacting Partners of a
Transcription Factor, YB-1 |
| title_full_unstemmed | The study of Interacting Partners of a
Transcription Factor, YB-1 |
| title_short | The study of Interacting Partners of a
Transcription Factor, YB-1 |
| title_sort | study of interacting partners of a
transcription factor, yb-1 |
| topic | RC Internal medicine |
| url | http://eprints.usm.my/47843/ http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf |