Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity
This study was aimed to establish a method to produce in-house recombinant human DNA topoisomerase. The DNA topoisomerase I (Topo I) and DNA topoisomerase II (Topo II) coding regions were amplified from the cDNA of breast cancer cells, MDA-MB-231. Topo I and Topo II coding region were ligated int...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://eprints.usm.my/47831/ http://eprints.usm.my/47831/1/EXPRESSION%20OF%20RECOMBINANT%20HUMAN%20DNA.pdf%20cut.pdf |
| _version_ | 1848880987299119104 |
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| author | Chan, Mooi Kwai |
| author_facet | Chan, Mooi Kwai |
| author_sort | Chan, Mooi Kwai |
| building | USM Institutional Repository |
| collection | Online Access |
| description | This study was aimed to establish a method to produce in-house recombinant human DNA topoisomerase. The DNA topoisomerase I (Topo I) and DNA topoisomerase II (Topo II) coding regions were amplified from the cDNA of breast cancer cells, MDA-MB-231. Topo I and Topo II coding region were ligated into expression vector, pPICZ--A and pPICZ--C, respectively. Subsequently, the constructed recombinant vectors were transformed into four types of Pichia pastoris strain, X-33, GS115, SMD1168H and KM71H. PCR screening for recombinant clones was performed and followed by selection of multicopy clones using agar plates containing increasing concentrations of Zeocin. The selected multicopy clones were then induced for enzymes expression in a shake flask system. Topo I was expressed relatively higher in GS115 than other Pichia strains. |
| first_indexed | 2025-11-15T18:11:51Z |
| format | Thesis |
| id | usm-47831 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:11:51Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-478312020-10-28T09:23:17Z http://eprints.usm.my/47831/ Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity Chan, Mooi Kwai QP Physiology This study was aimed to establish a method to produce in-house recombinant human DNA topoisomerase. The DNA topoisomerase I (Topo I) and DNA topoisomerase II (Topo II) coding regions were amplified from the cDNA of breast cancer cells, MDA-MB-231. Topo I and Topo II coding region were ligated into expression vector, pPICZ--A and pPICZ--C, respectively. Subsequently, the constructed recombinant vectors were transformed into four types of Pichia pastoris strain, X-33, GS115, SMD1168H and KM71H. PCR screening for recombinant clones was performed and followed by selection of multicopy clones using agar plates containing increasing concentrations of Zeocin. The selected multicopy clones were then induced for enzymes expression in a shake flask system. Topo I was expressed relatively higher in GS115 than other Pichia strains. 2017-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47831/1/EXPRESSION%20OF%20RECOMBINANT%20HUMAN%20DNA.pdf%20cut.pdf Chan, Mooi Kwai (2017) Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | QP Physiology Chan, Mooi Kwai Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title | Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title_full | Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title_fullStr | Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title_full_unstemmed | Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title_short | Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity |
| title_sort | expression of recombinant human dna topoisomerase in pichia pastoris and analysis of its activity |
| topic | QP Physiology |
| url | http://eprints.usm.my/47831/ http://eprints.usm.my/47831/1/EXPRESSION%20OF%20RECOMBINANT%20HUMAN%20DNA.pdf%20cut.pdf |