PCR-RDB assay for detection of vibrio cholerae
A colorimetric detection method has been designed and optimized by immobilizing a specific DNA probe onto Biodyne C membrane and hybridize it with heat denatured biotin labeled PCR amplicon to detect the presence of the hemM gene of Vibrio cholerae. Therefore it can be used to replaced agarose g...
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| Format: | Thesis |
| Language: | English |
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Universiti Sains Malaysia
2005
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| Online Access: | http://eprints.usm.my/47694/ http://eprints.usm.my/47694/1/PTA...Foong%20Sui%20Yun...2005...mka.-24%20pages.pdf |
| _version_ | 1848880948281606144 |
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| author | Yun, Foong Sui |
| author_facet | Yun, Foong Sui |
| author_sort | Yun, Foong Sui |
| building | USM Institutional Repository |
| collection | Online Access |
| description | A colorimetric detection method has been designed and optimized by
immobilizing a specific DNA probe onto Biodyne C membrane and hybridize it
with heat denatured biotin labeled PCR amplicon to detect the presence of the
hemM gene of Vibrio cholerae. Therefore it can be used to replaced agarose
gel electrophoresis and conventional culture method which are very low in
sensitivity for diagnosis of cholera. This method was refered to as PCR-RDB
assay. Optimization of the PCR-Rerverse Dot Blot assay include hybridization
temperature, amount of PCR amplicon, timing of the assay, optimizing of biotin
incorporation in PCR amplicon, dilution of streptavidin-alkaline phosphatase
conjugate used and development of a control positive for the assay. PCR-RDB
assay can be used in detection of biological or environmental samples to help in
control of cholera cases in high incidence area. |
| first_indexed | 2025-11-15T18:11:14Z |
| format | Thesis |
| id | usm-47694 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:11:14Z |
| publishDate | 2005 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-476942020-10-22T06:55:35Z http://eprints.usm.my/47694/ PCR-RDB assay for detection of vibrio cholerae Yun, Foong Sui R Medicine A colorimetric detection method has been designed and optimized by immobilizing a specific DNA probe onto Biodyne C membrane and hybridize it with heat denatured biotin labeled PCR amplicon to detect the presence of the hemM gene of Vibrio cholerae. Therefore it can be used to replaced agarose gel electrophoresis and conventional culture method which are very low in sensitivity for diagnosis of cholera. This method was refered to as PCR-RDB assay. Optimization of the PCR-Rerverse Dot Blot assay include hybridization temperature, amount of PCR amplicon, timing of the assay, optimizing of biotin incorporation in PCR amplicon, dilution of streptavidin-alkaline phosphatase conjugate used and development of a control positive for the assay. PCR-RDB assay can be used in detection of biological or environmental samples to help in control of cholera cases in high incidence area. Universiti Sains Malaysia 2005 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47694/1/PTA...Foong%20Sui%20Yun...2005...mka.-24%20pages.pdf Yun, Foong Sui (2005) PCR-RDB assay for detection of vibrio cholerae. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R Medicine Yun, Foong Sui PCR-RDB assay for detection of vibrio cholerae |
| title | PCR-RDB assay for detection of vibrio cholerae |
| title_full | PCR-RDB assay for detection of vibrio cholerae |
| title_fullStr | PCR-RDB assay for detection of vibrio cholerae |
| title_full_unstemmed | PCR-RDB assay for detection of vibrio cholerae |
| title_short | PCR-RDB assay for detection of vibrio cholerae |
| title_sort | pcr-rdb assay for detection of vibrio cholerae |
| topic | R Medicine |
| url | http://eprints.usm.my/47694/ http://eprints.usm.my/47694/1/PTA...Foong%20Sui%20Yun...2005...mka.-24%20pages.pdf |