IGFBP-2 in cervical cancer development
Objective: Increased expression of insulin-like growth factor binding protein 2, IGFBP-2, is associated with many cancers, though its role in cervical cancer is unclear. The aim of this study was to investigate the expression of IGFBP-2 protein and the transcriptomics profile of genes involved in...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Press
2020
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/47617/ http://eprints.usm.my/47617/1/IGFBP2%20Exp%20Mol%20Pathol%202020.pdf |
| _version_ | 1848880926776360960 |
|---|---|
| author | Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee |
| author_facet | Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee |
| author_sort | Kaur, Gurjeet |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Objective: Increased expression of insulin-like growth factor binding protein 2, IGFBP-2, is associated with many
cancers, though its role in cervical cancer is unclear. The aim of this study was to investigate the expression of
IGFBP-2 protein and the transcriptomics profile of genes involved in the IGF signaling pathway during cervical
cancer development.
Design: Immunohistochemical expression of IGFBP-2 protein was semi-quantitatively assessed in tissue microarrays
containing 9 normal cervix, 10 low grade cervical intraepithelial neoplasia (LGCIN), 10 high grade cervical
intraepithelial neoplasia (HGCIN) and 42 squamous cell carcinoma (SCC) cases. The gene expression
profiles of IGFBP-2, IGF-1, IGF-1R, PTEN, MDM2, AKT1 and TP53 were determined in three cervical tissue
samples each from normal cervix, human papillomavirus (HPV)-infected LGCIN, HGCIN and SCC, using Human
Transcriptome Array 2.0.
Results: IGFBP-2 protein was highly expressed in the cytoplasm of SCC cells compared to normal cervix
(p = .013). The expression was not significantly associated with CIN grade or SCC stage. Transcriptomics
profiling demonstrated upregulation of IGFBP-2 and TP53 in HGCIN and SCC compared to normal cervix. IGF-1,
IGF-1R and PTEN genes were downregulated in all histological groups. IGF-1 gene was significantly downregulated
in SCC (p = .031), while PTEN gene was significantly downregulated in HGCIN (p = .012), compared
to normal cervix. MDM2 and AKT1 genes were downregulated in LGCIN and HGCIN, while upregulated in SCC.
Conclusion: In cervical carcinogenesis, IGFBP-2 appears to play an oncogenic role, probably through an IGFindependent
mechanism. |
| first_indexed | 2025-11-15T18:10:53Z |
| format | Article |
| id | usm-47617 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:10:53Z |
| publishDate | 2020 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-476172020-10-30T20:48:41Z http://eprints.usm.my/47617/ IGFBP-2 in cervical cancer development Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Objective: Increased expression of insulin-like growth factor binding protein 2, IGFBP-2, is associated with many cancers, though its role in cervical cancer is unclear. The aim of this study was to investigate the expression of IGFBP-2 protein and the transcriptomics profile of genes involved in the IGF signaling pathway during cervical cancer development. Design: Immunohistochemical expression of IGFBP-2 protein was semi-quantitatively assessed in tissue microarrays containing 9 normal cervix, 10 low grade cervical intraepithelial neoplasia (LGCIN), 10 high grade cervical intraepithelial neoplasia (HGCIN) and 42 squamous cell carcinoma (SCC) cases. The gene expression profiles of IGFBP-2, IGF-1, IGF-1R, PTEN, MDM2, AKT1 and TP53 were determined in three cervical tissue samples each from normal cervix, human papillomavirus (HPV)-infected LGCIN, HGCIN and SCC, using Human Transcriptome Array 2.0. Results: IGFBP-2 protein was highly expressed in the cytoplasm of SCC cells compared to normal cervix (p = .013). The expression was not significantly associated with CIN grade or SCC stage. Transcriptomics profiling demonstrated upregulation of IGFBP-2 and TP53 in HGCIN and SCC compared to normal cervix. IGF-1, IGF-1R and PTEN genes were downregulated in all histological groups. IGF-1 gene was significantly downregulated in SCC (p = .031), while PTEN gene was significantly downregulated in HGCIN (p = .012), compared to normal cervix. MDM2 and AKT1 genes were downregulated in LGCIN and HGCIN, while upregulated in SCC. Conclusion: In cervical carcinogenesis, IGFBP-2 appears to play an oncogenic role, probably through an IGFindependent mechanism. Academic Press 2020 Article PeerReviewed application/pdf en http://eprints.usm.my/47617/1/IGFBP2%20Exp%20Mol%20Pathol%202020.pdf Kaur, Gurjeet and Balasubramaniam, Shandra Devi and Yung, Jen Lee (2020) IGFBP-2 in cervical cancer development. Experimental and Molecular Pathology, 113. p. 104362. ISSN 0014-4800 https://doi.org/10.1016/j.yexmp.2019.104362 |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee IGFBP-2 in cervical cancer development |
| title | IGFBP-2 in cervical cancer development |
| title_full | IGFBP-2 in cervical cancer development |
| title_fullStr | IGFBP-2 in cervical cancer development |
| title_full_unstemmed | IGFBP-2 in cervical cancer development |
| title_short | IGFBP-2 in cervical cancer development |
| title_sort | igfbp-2 in cervical cancer development |
| topic | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/47617/ http://eprints.usm.my/47617/ http://eprints.usm.my/47617/1/IGFBP2%20Exp%20Mol%20Pathol%202020.pdf |