Cloning and characterization of mycobacterium tuberculosis katG gene
katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major drug used in the treabnent of tuberculosis...
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| Format: | Article |
| Language: | English |
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katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major
2005
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| Online Access: | http://eprints.usm.my/47232/ http://eprints.usm.my/47232/1/PTA...Eugene%20Ray%20Martin...2005...PS..-24%20pages.pdf |
| _version_ | 1848880815106162688 |
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| author | Martin, Eugene Ray |
| author_facet | Martin, Eugene Ray |
| author_sort | Martin, Eugene Ray |
| building | USM Institutional Repository |
| collection | Online Access |
| description | katG is tbe sole gene encoding for catalase peroxidase enzyme in
Mycobacterium tuberculosis enabling its survival in the host macrophage. This same
gene has been indicted to play an important role in forming the killing effect of
isoniazid (INH), a major drug used in the treabnent of tuberculosis (TB)~ It has been
indicated that the folDlation of new generation drugs for TB will be connected to
further understanding this interaction and the study of the structures of the katG
gene.
The high molecular weight genomic DNA of Mycobacterium tubercuJ.o.,;., of
the clinical strain MTB69/03 from HUSM has successfully been isolated tbroogb a
modification of the method in D. van Soolingen et. al .. Two primers with extra sites
for tbe excision sites of EcoRl and Hind/11 were successfully used to produce a
DNA product of 2252 base pairs. The product was then inserted into a pCR®.2.lTOPO
® plasmid vector. The insert was continued by the use of both PCR and
restriction enzymes.
Tite successfully confinned inserted plasmids were then sent for sequencing. |
| first_indexed | 2025-11-15T18:09:07Z |
| format | Article |
| id | usm-47232 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:09:07Z |
| publishDate | 2005 |
| publisher | katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-472322020-09-13T03:28:31Z http://eprints.usm.my/47232/ Cloning and characterization of mycobacterium tuberculosis katG gene Martin, Eugene Ray RC306-320.5 Tuberculosis katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major drug used in the treabnent of tuberculosis (TB)~ It has been indicated that the folDlation of new generation drugs for TB will be connected to further understanding this interaction and the study of the structures of the katG gene. The high molecular weight genomic DNA of Mycobacterium tubercuJ.o.,;., of the clinical strain MTB69/03 from HUSM has successfully been isolated tbroogb a modification of the method in D. van Soolingen et. al .. Two primers with extra sites for tbe excision sites of EcoRl and Hind/11 were successfully used to produce a DNA product of 2252 base pairs. The product was then inserted into a pCR®.2.lTOPO ® plasmid vector. The insert was continued by the use of both PCR and restriction enzymes. Tite successfully confinned inserted plasmids were then sent for sequencing. katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major 2005 Article NonPeerReviewed application/pdf en http://eprints.usm.my/47232/1/PTA...Eugene%20Ray%20Martin...2005...PS..-24%20pages.pdf Martin, Eugene Ray (2005) Cloning and characterization of mycobacterium tuberculosis katG gene. katG is tbe sole gene encoding for catalase peroxidase enzyme in Mycobacterium tuberculosis enabling its survival in the host macrophage. This same gene has been indicted to play an important role in forming the killing effect of isoniazid (INH), a major. (Submitted) |
| spellingShingle | RC306-320.5 Tuberculosis Martin, Eugene Ray Cloning and characterization of mycobacterium tuberculosis katG gene |
| title | Cloning and characterization of mycobacterium tuberculosis katG gene |
| title_full | Cloning and characterization of mycobacterium tuberculosis katG gene |
| title_fullStr | Cloning and characterization of mycobacterium tuberculosis katG gene |
| title_full_unstemmed | Cloning and characterization of mycobacterium tuberculosis katG gene |
| title_short | Cloning and characterization of mycobacterium tuberculosis katG gene |
| title_sort | cloning and characterization of mycobacterium tuberculosis katg gene |
| topic | RC306-320.5 Tuberculosis |
| url | http://eprints.usm.my/47232/ http://eprints.usm.my/47232/1/PTA...Eugene%20Ray%20Martin...2005...PS..-24%20pages.pdf |