Fermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactor
In this work, fermentation was conducted for the feedstock and purification of lipase by Burkholderia metallica using a column reactor. The targeted parameter for this research such as salt stability, types and concentrations of polyethylene glycol(PEG) and salt, pH of extraction solution and flo...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Teknologi Industri
2020
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| Online Access: | http://eprints.usm.my/46948/ http://eprints.usm.my/46948/1/FERMENTATION%20AND%20PURIFICATION%20OF%20LIPASE%20%28ABSTRACT%29.pdf |
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| author | Ezzreen Zafhrina Mohamad Redza, Nur |
| author_facet | Ezzreen Zafhrina Mohamad Redza, Nur |
| author_sort | Ezzreen Zafhrina Mohamad Redza, Nur |
| building | USM Institutional Repository |
| collection | Online Access |
| description | In this work, fermentation was conducted for the feedstock and purification of lipase by
Burkholderia metallica using a column reactor. The targeted parameter for this research such as
salt stability, types and concentrations of polyethylene glycol(PEG) and salt, pH of extraction
solution and flowrate ml/s to see the performance of extractive purification system. Aqueous
impregnated resin system(AIRS) is applied using porous glass beads grafted with polyethylene
glycol (PEG). An evaluation studies on AIRS were performed with various molecular weight of
polyethylene glycol (PEG) (PEG 2,000, PEG 4,000, PEG 6,000) and different type of salts
(sodium citrate, potassium citrate and sodium acetate) as phase component. Liquid extractant of
PEG was immobilized on the inert support (porous glass beads) and correspond to the aqueous
state phase to surmount the drawback of aqueous two phase extraction (ATPE). The technique
combines liquid-liquid extraction with ease if operation of column chromatography. |
| first_indexed | 2025-11-15T18:07:55Z |
| format | Monograph |
| id | usm-46948 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:07:55Z |
| publishDate | 2020 |
| publisher | Pusat Pengajian Teknologi Industri |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-469482020-08-27T01:44:58Z http://eprints.usm.my/46948/ Fermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactor Ezzreen Zafhrina Mohamad Redza, Nur T1-995 Technology(General) In this work, fermentation was conducted for the feedstock and purification of lipase by Burkholderia metallica using a column reactor. The targeted parameter for this research such as salt stability, types and concentrations of polyethylene glycol(PEG) and salt, pH of extraction solution and flowrate ml/s to see the performance of extractive purification system. Aqueous impregnated resin system(AIRS) is applied using porous glass beads grafted with polyethylene glycol (PEG). An evaluation studies on AIRS were performed with various molecular weight of polyethylene glycol (PEG) (PEG 2,000, PEG 4,000, PEG 6,000) and different type of salts (sodium citrate, potassium citrate and sodium acetate) as phase component. Liquid extractant of PEG was immobilized on the inert support (porous glass beads) and correspond to the aqueous state phase to surmount the drawback of aqueous two phase extraction (ATPE). The technique combines liquid-liquid extraction with ease if operation of column chromatography. Pusat Pengajian Teknologi Industri 2020-06 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/46948/1/FERMENTATION%20AND%20PURIFICATION%20OF%20LIPASE%20%28ABSTRACT%29.pdf Ezzreen Zafhrina Mohamad Redza, Nur (2020) Fermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactor. Project Report. Pusat Pengajian Teknologi Industri. (Submitted) http://ethesis.usm.my:8080/jspui/ |
| spellingShingle | T1-995 Technology(General) Ezzreen Zafhrina Mohamad Redza, Nur Fermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactor |
| title | Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor |
| title_full | Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor |
| title_fullStr | Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor |
| title_full_unstemmed | Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor |
| title_short | Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor |
| title_sort | fermentation and purification of lipase
by burkholderia metallica
using column reactor |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/46948/ http://eprints.usm.my/46948/ http://eprints.usm.my/46948/1/FERMENTATION%20AND%20PURIFICATION%20OF%20LIPASE%20%28ABSTRACT%29.pdf |