Antibacterial activity of some selected brands of Malaysian honey

Honey is an ancient remedy for the treatment of infected wounds, which has recently been 'rediscovered' by the medical profession, particularly where conventional modern therapeutic agents are failing. Honey is made up of simple sugars and is an excellent source of energy. It is hyperto...

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Main Author: Tumin, Norizah
Format: Thesis
Language:English
Published: 2004
Subjects:
Online Access:http://eprints.usm.my/46332/
http://eprints.usm.my/46332/1/LI...Norizah%20Binti%20Tumin...2004...-24%20pages.pdf
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author Tumin, Norizah
author_facet Tumin, Norizah
author_sort Tumin, Norizah
building USM Institutional Repository
collection Online Access
description Honey is an ancient remedy for the treatment of infected wounds, which has recently been 'rediscovered' by the medical profession, particularly where conventional modern therapeutic agents are failing. Honey is made up of simple sugars and is an excellent source of energy. It is hypertonic and sterile with antibacterial activity against many pathogenic bacteria. The present study aims to investigate the antibacterial activities of five different brands of Malaysian honey. 'Tualang', 'Hutan' and 'Gelang' honey are produced from wild bee, 'Pucuk Daun' and 'Ee Feng Gu' honey are from honeybee farm were considered for this study. Six different pathogenic bacteria, E. coli, S. typhi, S. sonnei, P. aeruginosa, S. aureus and S. pyogenes were selected as test organisms. Diluted and neat honeys were used for the antibacterial assay. Solvent extraction of honey was carried out with an aim to isolate the active principle behind the antibacterial property. MIC of the selected honey was determined. 'Tualang' honey was found to be most effective against S. typhi. It also showed a substantial antibacterial activity against E. coli and S. pyogenes. 'Hutan' and 'Gelang' honey does not show any antibacterial activity against the organism tested. 'Pucuk Daun' honey showed antibacterial activity E. coli and S. sonnei. 'Ee Feng Gu' was active against E. coli, S. typhi, S. sonnei and S. aureus. Among the solvent extracts only acetone fraction showed antibacterial activity. Extracts with petroleum ether, chloroform and ethyl acetate does not show any activity. The MIC (Minimum Inhibitory Concentration) values of 'Pucuk Daun', 'Gelang' and 'Hutan' honey were 5.0 IJQ/ml, 17.5 IJQ/ml and 28.75 IJQ/ml respectively against S. sonnei.
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language English
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spelling usm-463322020-02-26T01:46:06Z http://eprints.usm.my/46332/ Antibacterial activity of some selected brands of Malaysian honey Tumin, Norizah RA Public aspects of medicine Honey is an ancient remedy for the treatment of infected wounds, which has recently been 'rediscovered' by the medical profession, particularly where conventional modern therapeutic agents are failing. Honey is made up of simple sugars and is an excellent source of energy. It is hypertonic and sterile with antibacterial activity against many pathogenic bacteria. The present study aims to investigate the antibacterial activities of five different brands of Malaysian honey. 'Tualang', 'Hutan' and 'Gelang' honey are produced from wild bee, 'Pucuk Daun' and 'Ee Feng Gu' honey are from honeybee farm were considered for this study. Six different pathogenic bacteria, E. coli, S. typhi, S. sonnei, P. aeruginosa, S. aureus and S. pyogenes were selected as test organisms. Diluted and neat honeys were used for the antibacterial assay. Solvent extraction of honey was carried out with an aim to isolate the active principle behind the antibacterial property. MIC of the selected honey was determined. 'Tualang' honey was found to be most effective against S. typhi. It also showed a substantial antibacterial activity against E. coli and S. pyogenes. 'Hutan' and 'Gelang' honey does not show any antibacterial activity against the organism tested. 'Pucuk Daun' honey showed antibacterial activity E. coli and S. sonnei. 'Ee Feng Gu' was active against E. coli, S. typhi, S. sonnei and S. aureus. Among the solvent extracts only acetone fraction showed antibacterial activity. Extracts with petroleum ether, chloroform and ethyl acetate does not show any activity. The MIC (Minimum Inhibitory Concentration) values of 'Pucuk Daun', 'Gelang' and 'Hutan' honey were 5.0 IJQ/ml, 17.5 IJQ/ml and 28.75 IJQ/ml respectively against S. sonnei. 2004 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46332/1/LI...Norizah%20Binti%20Tumin...2004...-24%20pages.pdf Tumin, Norizah (2004) Antibacterial activity of some selected brands of Malaysian honey. Other thesis, Universiti Sains Malaysia.
spellingShingle RA Public aspects of medicine
Tumin, Norizah
Antibacterial activity of some selected brands of Malaysian honey
title Antibacterial activity of some selected brands of Malaysian honey
title_full Antibacterial activity of some selected brands of Malaysian honey
title_fullStr Antibacterial activity of some selected brands of Malaysian honey
title_full_unstemmed Antibacterial activity of some selected brands of Malaysian honey
title_short Antibacterial activity of some selected brands of Malaysian honey
title_sort antibacterial activity of some selected brands of malaysian honey
topic RA Public aspects of medicine
url http://eprints.usm.my/46332/
http://eprints.usm.my/46332/1/LI...Norizah%20Binti%20Tumin...2004...-24%20pages.pdf