Establishment of multiplex PCR for the detection of common neurotropic viruses.
Viral infections of the central nervous system (CNS) may result in clinical syndromes like aseptic meningitis, encephalitis and myelitis. These infections are often difficult to diagnose using conventional laboratory techniques like viral culture and serology. These methods are also time consumi...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2005
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| Online Access: | http://eprints.usm.my/45984/ http://eprints.usm.my/45984/1/GP...Establishment%20Of%20Multiplex%20PCR%20For%20The%20Detection%20Of%20Common%20Neurotropic%20Viruses...2005...-24%20pages.pdf |
| _version_ | 1848880480630341632 |
|---|---|
| author | Gopalakrishnan, V. |
| author_facet | Gopalakrishnan, V. |
| author_sort | Gopalakrishnan, V. |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Viral infections of the central nervous system (CNS) may result in
clinical syndromes like aseptic meningitis, encephalitis and myelitis.
These infections are often difficult to diagnose using conventional
laboratory techniques like viral culture and serology. These
methods are also time consuming and unsatisfactory. Hence a
study was designed to develop a rapid technique to detect the viral
etiology. In this study a reverse transcriptase (RT) multiplex PCR to
detect viral etiologies in CNS infections was standardized. The RT
multiplex PCR was designed to detect enterovirus, herpes simplex
and varicella zoster viruses. Three sets of primers were employed
for their detection. Amplification of target sequences was
qualitatively analyzed by looking for the presence or absence of
amplicons on a 2o/o agarose gel stained with ethidium bromide.
Sensitivity of the PCR has been ascertained. Further, 3 sets of
primers were used to perform a second PCR (nested), which
confirms the product specificity, and also helps in increasing the
sensitivity of the assay. The RT multiplex PCR standardized can be
employed to detect herpes, varicella and enteroviral infections. |
| first_indexed | 2025-11-15T18:03:48Z |
| format | Article |
| id | usm-45984 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:03:48Z |
| publishDate | 2005 |
| publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-459842020-01-23T03:50:46Z http://eprints.usm.my/45984/ Establishment of multiplex PCR for the detection of common neurotropic viruses. Gopalakrishnan, V. RA643-645 Disease (Communicable and noninfectious) and public health Viral infections of the central nervous system (CNS) may result in clinical syndromes like aseptic meningitis, encephalitis and myelitis. These infections are often difficult to diagnose using conventional laboratory techniques like viral culture and serology. These methods are also time consuming and unsatisfactory. Hence a study was designed to develop a rapid technique to detect the viral etiology. In this study a reverse transcriptase (RT) multiplex PCR to detect viral etiologies in CNS infections was standardized. The RT multiplex PCR was designed to detect enterovirus, herpes simplex and varicella zoster viruses. Three sets of primers were employed for their detection. Amplification of target sequences was qualitatively analyzed by looking for the presence or absence of amplicons on a 2o/o agarose gel stained with ethidium bromide. Sensitivity of the PCR has been ascertained. Further, 3 sets of primers were used to perform a second PCR (nested), which confirms the product specificity, and also helps in increasing the sensitivity of the assay. The RT multiplex PCR standardized can be employed to detect herpes, varicella and enteroviral infections. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2005-03 Article NonPeerReviewed application/pdf en http://eprints.usm.my/45984/1/GP...Establishment%20Of%20Multiplex%20PCR%20For%20The%20Detection%20Of%20Common%20Neurotropic%20Viruses...2005...-24%20pages.pdf Gopalakrishnan, V. (2005) Establishment of multiplex PCR for the detection of common neurotropic viruses. Establishment of multiplex PCR for the detection of common neurotropic viruses'. (Submitted) |
| spellingShingle | RA643-645 Disease (Communicable and noninfectious) and public health Gopalakrishnan, V. Establishment of multiplex PCR for the detection of common neurotropic viruses. |
| title | Establishment of multiplex PCR for the detection of common
neurotropic viruses. |
| title_full | Establishment of multiplex PCR for the detection of common
neurotropic viruses. |
| title_fullStr | Establishment of multiplex PCR for the detection of common
neurotropic viruses. |
| title_full_unstemmed | Establishment of multiplex PCR for the detection of common
neurotropic viruses. |
| title_short | Establishment of multiplex PCR for the detection of common
neurotropic viruses. |
| title_sort | establishment of multiplex pcr for the detection of common
neurotropic viruses. |
| topic | RA643-645 Disease (Communicable and noninfectious) and public health |
| url | http://eprints.usm.my/45984/ http://eprints.usm.my/45984/1/GP...Establishment%20Of%20Multiplex%20PCR%20For%20The%20Detection%20Of%20Common%20Neurotropic%20Viruses...2005...-24%20pages.pdf |