Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | http://eprints.usm.my/45006/ http://eprints.usm.my/45006/1/LEONG%20YIN%20YEE.pdf |
| _version_ | 1848880210952323072 |
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| author | Leong, Yin Yee |
| author_facet | Leong, Yin Yee |
| author_sort | Leong, Yin Yee |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This study aimed to examine the synergistic effects of MSCs on CSC cardiomyocyte differentiation. Methods: C-kit CSCs and MSCs were isolated from 4-6 weeks C57BL/6N mice (n=9). CSC cardiomyocyte differentiation was tested after re-isolated from MSC co-culture using micron-sized particles of iron oxide, and compared to CSCs treated with MSC-conditioned medium (CdM) or without any treatment. |
| first_indexed | 2025-11-15T17:59:31Z |
| format | Thesis |
| id | usm-45006 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:59:31Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-450062019-07-23T00:56:18Z http://eprints.usm.my/45006/ Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro Leong, Yin Yee R735-854 Medical education. Medical schools. Research Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This study aimed to examine the synergistic effects of MSCs on CSC cardiomyocyte differentiation. Methods: C-kit CSCs and MSCs were isolated from 4-6 weeks C57BL/6N mice (n=9). CSC cardiomyocyte differentiation was tested after re-isolated from MSC co-culture using micron-sized particles of iron oxide, and compared to CSCs treated with MSC-conditioned medium (CdM) or without any treatment. 2016-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45006/1/LEONG%20YIN%20YEE.pdf Leong, Yin Yee (2016) Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Leong, Yin Yee Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title | Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title_full | Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title_fullStr | Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title_full_unstemmed | Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title_short | Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro |
| title_sort | interaction between murine cardiac stem cells and bone marrow-derived mesenchymal stem cells for cardiomyocyte differentiation in vitro |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/45006/ http://eprints.usm.my/45006/1/LEONG%20YIN%20YEE.pdf |