Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/44994/ http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf |
| _version_ | 1848880208168353792 |
|---|---|
| author | Marimuthu, Citartan |
| author_facet | Marimuthu, Citartan |
| author_sort | Marimuthu, Citartan |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully isolated by SELEX technology. After 11 cycles of SELEX, cloning and sequence analysis showed an appearance of a single major clone constituting the putative RNA aptamer, termed REPORA-6, with a dissociation constant of 25±1 nM. |
| first_indexed | 2025-11-15T17:59:28Z |
| format | Thesis |
| id | usm-44994 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:59:28Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-449942019-07-22T03:03:01Z http://eprints.usm.my/44994/ Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology Marimuthu, Citartan R735-854 Medical education. Medical schools. Research Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully isolated by SELEX technology. After 11 cycles of SELEX, cloning and sequence analysis showed an appearance of a single major clone constituting the putative RNA aptamer, termed REPORA-6, with a dissociation constant of 25±1 nM. 2014-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf Marimuthu, Citartan (2014) Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Marimuthu, Citartan Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title | Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title_full | Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title_fullStr | Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title_full_unstemmed | Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title_short | Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology |
| title_sort | generation and characterization of rna aptamer against rhuepo-a by selex technology |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/44994/ http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf |