Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be in...
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| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | http://eprints.usm.my/44885/ http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf |
| _version_ | 1848880180764868608 |
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| author | Ishak, Rafidah |
| author_facet | Ishak, Rafidah |
| author_sort | Ishak, Rafidah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures. |
| first_indexed | 2025-11-15T17:59:02Z |
| format | Thesis |
| id | usm-44885 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:59:02Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-448852019-07-05T07:42:57Z http://eprints.usm.my/44885/ Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids Ishak, Rafidah QH1 Natural history (General - Including nature conservation, geographical distribution) Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures. 2012-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf Ishak, Rafidah (2012) Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Ishak, Rafidah Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title | Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title_full | Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title_fullStr | Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title_full_unstemmed | Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title_short | Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids |
| title_sort | establishment of cell suspension culture of hyoscyamus niger l. for the production of tropane alkaloids |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/44885/ http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf |