Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation
The initial discovery of microbial transglutaminase (mTGase) was to soothe the high cost of mammalian transglutaminase in food processing industries. This enzyme has become an important tool in biotechnological applications due to its robustness, availability in high purity, bypass the need of calci...
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| Format: | Thesis |
| Language: | English |
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2018
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| Online Access: | http://eprints.usm.my/44117/ http://eprints.usm.my/44117/1/CHAN%20SOO%20KHIM.pdf |
| _version_ | 1848879979215978496 |
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| author | Chan, Soo Khim |
| author_facet | Chan, Soo Khim |
| author_sort | Chan, Soo Khim |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The initial discovery of microbial transglutaminase (mTGase) was to soothe the high cost of mammalian transglutaminase in food processing industries. This enzyme has become an important tool in biotechnological applications due to its robustness, availability in high purity, bypass the need of calcium ions for activation and high reaction rate. However, mTGase has low substrate specificity due to its wide active site cleft which impeding its diverse applications in biotechnological use. Therefore, mutagenesis of mTGase to obtain substrate specific enzyme is highly desirable for site-specific conjugations. |
| first_indexed | 2025-11-15T17:55:50Z |
| format | Thesis |
| id | usm-44117 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:55:50Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-441172019-04-18T01:48:13Z http://eprints.usm.my/44117/ Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation Chan, Soo Khim R735-854 Medical education. Medical schools. Research The initial discovery of microbial transglutaminase (mTGase) was to soothe the high cost of mammalian transglutaminase in food processing industries. This enzyme has become an important tool in biotechnological applications due to its robustness, availability in high purity, bypass the need of calcium ions for activation and high reaction rate. However, mTGase has low substrate specificity due to its wide active site cleft which impeding its diverse applications in biotechnological use. Therefore, mutagenesis of mTGase to obtain substrate specific enzyme is highly desirable for site-specific conjugations. 2018-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44117/1/CHAN%20SOO%20KHIM.pdf Chan, Soo Khim (2018) Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Chan, Soo Khim Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title | Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title_full | Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title_fullStr | Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title_full_unstemmed | Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title_short | Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation |
| title_sort | mutagenesis of microbial transglutaminase for qtag-ktag conjugation |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/44117/ http://eprints.usm.my/44117/1/CHAN%20SOO%20KHIM.pdf |