Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules

Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the anti-candidal activity and toxicity of Alginate/EUDRAGIT® S 100-enclosed chitosan–calcium phosphate-loaded Fe-bLf nanocapsules (NCs) by in vitro and in vivo study. The screening of NCs for an...

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Main Author: Khoo, Miew Leng
Format: Thesis
Language:English
Published: 2018
Subjects:
Online Access:http://eprints.usm.my/44020/
http://eprints.usm.my/44020/1/KHOO%20MIEW%20LENG.pdf
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author Khoo, Miew Leng
author_facet Khoo, Miew Leng
author_sort Khoo, Miew Leng
building USM Institutional Repository
collection Online Access
description Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the anti-candidal activity and toxicity of Alginate/EUDRAGIT® S 100-enclosed chitosan–calcium phosphate-loaded Fe-bLf nanocapsules (NCs) by in vitro and in vivo study. The screening of NCs for antimicrobial activity showed that NCs has good anti-candidal activity. The minimum inhibitory concentration (MIC) value of NCs using broth microdilution method was found to be 500 μg/mL. The growth profile study demonstrated that the NCs at MIC, two times MIC and four times MIC inhibited the growth of Candida albicans USM-K1 (C. albicans). The electron microscopy studies showed that NCs caused the destruction of yeast cells by disruption of cell wall and cell membrane with increase of incubation time until 36 hours.
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institution Universiti Sains Malaysia
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language English
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publishDate 2018
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spelling usm-440202019-04-12T05:24:50Z http://eprints.usm.my/44020/ Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules Khoo, Miew Leng R735-854 Medical education. Medical schools. Research Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the anti-candidal activity and toxicity of Alginate/EUDRAGIT® S 100-enclosed chitosan–calcium phosphate-loaded Fe-bLf nanocapsules (NCs) by in vitro and in vivo study. The screening of NCs for antimicrobial activity showed that NCs has good anti-candidal activity. The minimum inhibitory concentration (MIC) value of NCs using broth microdilution method was found to be 500 μg/mL. The growth profile study demonstrated that the NCs at MIC, two times MIC and four times MIC inhibited the growth of Candida albicans USM-K1 (C. albicans). The electron microscopy studies showed that NCs caused the destruction of yeast cells by disruption of cell wall and cell membrane with increase of incubation time until 36 hours. 2018-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44020/1/KHOO%20MIEW%20LENG.pdf Khoo, Miew Leng (2018) Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules. PhD thesis, Universiti Sains Malaysia.
spellingShingle R735-854 Medical education. Medical schools. Research
Khoo, Miew Leng
Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title_full Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title_fullStr Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title_full_unstemmed Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title_short Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules
title_sort anti-candidal activity and toxicity studies of alginate-enclosed chitosan–calcium phosphate-loaded fe-bovine lactoferrin nanocapsules
topic R735-854 Medical education. Medical schools. Research
url http://eprints.usm.my/44020/
http://eprints.usm.my/44020/1/KHOO%20MIEW%20LENG.pdf