Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consu...
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| Format: | Thesis |
| Language: | English |
| Published: |
2018
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| Online Access: | http://eprints.usm.my/43993/ http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf |
| _version_ | 1848879947924373504 |
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| author | Manaf, Normaliza Abdul |
| author_facet | Manaf, Normaliza Abdul |
| author_sort | Manaf, Normaliza Abdul |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consumed Tongkat Ali (TA) supplement using the solid phase extraction (SPE) and liquid-liquid extraction (LLE) method. From 47 samples, 11 samples violated the World Anti-Doping Agency (WADA) guidelines and confirmation by gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) showed 5 samples were consistent with the exogenous origin (Δδ13C > 3‰). Two microextraction methods, i.e., vortexassisted liquid-liquid microextraction (VALLME) and micro-solid phase extraction (μ-SPE) followed by LC-MS/MS were developed. Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm) with gradient elution resulted in baseline separation for compounds in about 8 min. |
| first_indexed | 2025-11-15T17:55:20Z |
| format | Thesis |
| id | usm-43993 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:55:20Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-439932019-04-12T05:24:50Z http://eprints.usm.my/43993/ Development Of New Analytical Methods For Steroid Profiling For Doping Purposes Manaf, Normaliza Abdul QD1-999 Chemistry The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consumed Tongkat Ali (TA) supplement using the solid phase extraction (SPE) and liquid-liquid extraction (LLE) method. From 47 samples, 11 samples violated the World Anti-Doping Agency (WADA) guidelines and confirmation by gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) showed 5 samples were consistent with the exogenous origin (Δδ13C > 3‰). Two microextraction methods, i.e., vortexassisted liquid-liquid microextraction (VALLME) and micro-solid phase extraction (μ-SPE) followed by LC-MS/MS were developed. Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm) with gradient elution resulted in baseline separation for compounds in about 8 min. 2018-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf Manaf, Normaliza Abdul (2018) Development Of New Analytical Methods For Steroid Profiling For Doping Purposes. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | QD1-999 Chemistry Manaf, Normaliza Abdul Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title | Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title_full | Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title_fullStr | Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title_full_unstemmed | Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title_short | Development Of New Analytical Methods For Steroid Profiling For Doping Purposes |
| title_sort | development of new analytical methods for steroid profiling for doping purposes |
| topic | QD1-999 Chemistry |
| url | http://eprints.usm.my/43993/ http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf |