Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia

In Malaysia, tobacco industry is very crucial in uplifting the socio-economic status of farmer in Kelantan and Terengganu. Root-knot nematodes (Meloidogyne spp.) lower the production and quality of tobacco. In this study, 24 tobaccos cultivation areas were surveyed for root gall disease and sp...

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Main Author: Ishak, Mohd Nurmuhaimin Adli
Format: Thesis
Language:English
Published: 2013
Subjects:
Online Access:http://eprints.usm.my/43826/
http://eprints.usm.my/43826/1/Mohd%20Nurmuhaimin%20Adli%20Bin%20Ishak24.pdf
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author Ishak, Mohd Nurmuhaimin Adli
author_facet Ishak, Mohd Nurmuhaimin Adli
author_sort Ishak, Mohd Nurmuhaimin Adli
building USM Institutional Repository
collection Online Access
description In Malaysia, tobacco industry is very crucial in uplifting the socio-economic status of farmer in Kelantan and Terengganu. Root-knot nematodes (Meloidogyne spp.) lower the production and quality of tobacco. In this study, 24 tobaccos cultivation areas were surveyed for root gall disease and species diversity of root- knot nematode. Soil physical and pH analysis were carried out to investigate their correlation to disease severity and number of root-knot nematode number in soil. Isolated Meloidogyne spp. was observed for morphology for genus identification and further analyses via Touchdown Polymerase chain reaction (TD-PCR) for species identification. From 24 cultivation area, 22 showed infestation of root-knot nematode. Tobacco plant infected with root-knot nematode showed root gall, yellowing leaves and stunted growth. One- Way ANOVA analysis showed that soil physical properties, and soil pH affect disease severity and Meloidogyne spp. number in soil. Meloidogyne spp. numbers in soil also affect disease severity. From Pearson correlation analysis, there was significant correlation at 0.01 between Meloidogyne spp. number in soil (r = 0.753), soil pH (r = 0.238), soil moisture (r = 0.203) and soil bulk density (r = 0.227) to disease severity.
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format Thesis
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institution Universiti Sains Malaysia
institution_category Local University
language English
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publishDate 2013
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spelling usm-438262019-04-12T05:26:18Z http://eprints.usm.my/43826/ Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia Ishak, Mohd Nurmuhaimin Adli QH1 Natural history (General - Including nature conservation, geographical distribution) In Malaysia, tobacco industry is very crucial in uplifting the socio-economic status of farmer in Kelantan and Terengganu. Root-knot nematodes (Meloidogyne spp.) lower the production and quality of tobacco. In this study, 24 tobaccos cultivation areas were surveyed for root gall disease and species diversity of root- knot nematode. Soil physical and pH analysis were carried out to investigate their correlation to disease severity and number of root-knot nematode number in soil. Isolated Meloidogyne spp. was observed for morphology for genus identification and further analyses via Touchdown Polymerase chain reaction (TD-PCR) for species identification. From 24 cultivation area, 22 showed infestation of root-knot nematode. Tobacco plant infected with root-knot nematode showed root gall, yellowing leaves and stunted growth. One- Way ANOVA analysis showed that soil physical properties, and soil pH affect disease severity and Meloidogyne spp. number in soil. Meloidogyne spp. numbers in soil also affect disease severity. From Pearson correlation analysis, there was significant correlation at 0.01 between Meloidogyne spp. number in soil (r = 0.753), soil pH (r = 0.238), soil moisture (r = 0.203) and soil bulk density (r = 0.227) to disease severity. 2013 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43826/1/Mohd%20Nurmuhaimin%20Adli%20Bin%20Ishak24.pdf Ishak, Mohd Nurmuhaimin Adli (2013) Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia. Masters thesis, Universiti Sains Malaysia.
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Ishak, Mohd Nurmuhaimin Adli
Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title_full Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title_fullStr Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title_full_unstemmed Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title_short Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia
title_sort soil physical factors affecting disease severity of root-knot nematode on tobacco and morphology and molecular identification of tropical nematodes in east coast malaysia
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
url http://eprints.usm.my/43826/
http://eprints.usm.my/43826/1/Mohd%20Nurmuhaimin%20Adli%20Bin%20Ishak24.pdf