Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies

Twenty percent of the genes from Klebsiella pneumonaie MGH78578 coded for hypothetical protein. Two particular hypothetical proteins KPN00728 and KPN00729 were identified using bioinformatics approaches. Both open reading frames showed high sequence homology to succinate dehydrogenase Chain C (SdhC)...

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Main Author: Choi, Sy Bing
Format: Thesis
Language:English
Published: 2011
Subjects:
Online Access:http://eprints.usm.my/43236/
http://eprints.usm.my/43236/1/CHOI%20SY%20BING.pdf
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author Choi, Sy Bing
author_facet Choi, Sy Bing
author_sort Choi, Sy Bing
building USM Institutional Repository
collection Online Access
description Twenty percent of the genes from Klebsiella pneumonaie MGH78578 coded for hypothetical protein. Two particular hypothetical proteins KPN00728 and KPN00729 were identified using bioinformatics approaches. Both open reading frames showed high sequence homology to succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escherichia coli KPN00729 was annotated as SdhD in May 2008. Thus, investigation on KPN00728 remained as no annotation for SdhC gene in the complete genome sequence of Klebsiella pneumoniae MGH78578. In this study, KPN00728 has a missing region with conserved residues which is important for ubiquinone (UQ) and heme group binding. Structure and function prediction of KPN00728 coupled with secondary structure analysis and transmembrane topology showed KPN00728 adopts SDH-(subunit C)-like structure.
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institution Universiti Sains Malaysia
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language English
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publishDate 2011
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spelling usm-432362019-04-12T05:26:29Z http://eprints.usm.my/43236/ Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies Choi, Sy Bing RS1-441 Pharmacy and materia medica Twenty percent of the genes from Klebsiella pneumonaie MGH78578 coded for hypothetical protein. Two particular hypothetical proteins KPN00728 and KPN00729 were identified using bioinformatics approaches. Both open reading frames showed high sequence homology to succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escherichia coli KPN00729 was annotated as SdhD in May 2008. Thus, investigation on KPN00728 remained as no annotation for SdhC gene in the complete genome sequence of Klebsiella pneumoniae MGH78578. In this study, KPN00728 has a missing region with conserved residues which is important for ubiquinone (UQ) and heme group binding. Structure and function prediction of KPN00728 coupled with secondary structure analysis and transmembrane topology showed KPN00728 adopts SDH-(subunit C)-like structure. 2011-10 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43236/1/CHOI%20SY%20BING.pdf Choi, Sy Bing (2011) Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies. PhD thesis, Universiti Sains Malaysia.
spellingShingle RS1-441 Pharmacy and materia medica
Choi, Sy Bing
Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title_full Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title_fullStr Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title_full_unstemmed Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title_short Structural And Functional Prediction Of Hypothetical Proteins From Klebsiella Pneumoniae MGH78578: Molecular Modelling Studies
title_sort structural and functional prediction of hypothetical proteins from klebsiella pneumoniae mgh78578: molecular modelling studies
topic RS1-441 Pharmacy and materia medica
url http://eprints.usm.my/43236/
http://eprints.usm.my/43236/1/CHOI%20SY%20BING.pdf