Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes...
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| Online Access: | http://eprints.usm.my/42635/ http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf |
| _version_ | 1848879613151805440 |
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| author | Rashid, Faridah Abdul Mustapha, Mohd Rafi |
| author_facet | Rashid, Faridah Abdul Mustapha, Mohd Rafi |
| author_sort | Rashid, Faridah Abdul |
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| description | Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes, DNA were extracted from huffy coat
amplified by PCR, restricted by endonuclease, electrophoresed on polyacrylamide gel and visualised by ethidium bromide staining. The genotypes obtained were: E-3/3(64%), E-4/3(27%), E-3/2(5%), and E-4/2(4%), and gene frequencies were: £2(0.04), e3(0.80), £4(0.16). The E-3/3 genotype contributed to hypercholesterolemia, low HDLC, high LDLC high ApoB. The E-4/2 genotype gave the opposite effect of E-3/3. The E-4/3 genotype exhibited hypertriglyceridemia, low LDLC and low ApoB. The E-3/2 genotype manifested normotriglyceridemia, high HDLC, low ApoA-I and low AlB ratio. We conclude that the E-3/3 genotype had the highest risk for premature heart disease. Those with the other genotypes (E-4/3, E-4/2 and E-3/2) had lower risk of premature heart disease. |
| first_indexed | 2025-11-15T17:50:01Z |
| format | Conference or Workshop Item |
| id | usm-42635 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
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| spelling | usm-426352020-03-25T09:51:28Z http://eprints.usm.my/42635/ Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease Rashid, Faridah Abdul Mustapha, Mohd Rafi RC666-701 Diseases of the circulatory (Cardiovascular) system Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes, DNA were extracted from huffy coat amplified by PCR, restricted by endonuclease, electrophoresed on polyacrylamide gel and visualised by ethidium bromide staining. The genotypes obtained were: E-3/3(64%), E-4/3(27%), E-3/2(5%), and E-4/2(4%), and gene frequencies were: £2(0.04), e3(0.80), £4(0.16). The E-3/3 genotype contributed to hypercholesterolemia, low HDLC, high LDLC high ApoB. The E-4/2 genotype gave the opposite effect of E-3/3. The E-4/3 genotype exhibited hypertriglyceridemia, low LDLC and low ApoB. The E-3/2 genotype manifested normotriglyceridemia, high HDLC, low ApoA-I and low AlB ratio. We conclude that the E-3/3 genotype had the highest risk for premature heart disease. Those with the other genotypes (E-4/3, E-4/2 and E-3/2) had lower risk of premature heart disease. Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf Rashid, Faridah Abdul and Mustapha, Mohd Rafi Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease. In: Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease, 8-9 June 1998, USM Kubang Kerian. (Submitted) |
| spellingShingle | RC666-701 Diseases of the circulatory (Cardiovascular) system Rashid, Faridah Abdul Mustapha, Mohd Rafi Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title | Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title_full | Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title_fullStr | Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title_full_unstemmed | Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title_short | Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease |
| title_sort | effect of apolipoprotein e genotype variation on the risk of premature ischaemic heart disease |
| topic | RC666-701 Diseases of the circulatory (Cardiovascular) system |
| url | http://eprints.usm.my/42635/ http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf |