(Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system
Proliferating cell nuclear antigen (PCNA) and Ki-67 are the two important molecules expressed by the proliferating cells. They are important during the DNA synthesis. We applied the antibodies (using DAKO EPOS system) against these two proteins to a series of 54 cases of colorectal adenocarcinoma of...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2001
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| Online Access: | http://eprints.usm.my/42546/ http://eprints.usm.my/42546/1/GP...Analisa_Eskperimen_PCNA_Dan_Ki-67_Sebagai_Petunjuk_Proliferasi_Sel_Dalam_Kes_Barah_Kolon_Di_HUSM_Dan_Hubungannya_Dengan_Duke%27s_Staging_System...2001...OCR...pdf |
| _version_ | 1848879589790580736 |
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| author | Kamal, Meor Zamari Meor Jaafar, Hasnan Ali, Kholip J, Rosli |
| author_facet | Kamal, Meor Zamari Meor Jaafar, Hasnan Ali, Kholip J, Rosli |
| author_sort | Kamal, Meor Zamari Meor |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Proliferating cell nuclear antigen (PCNA) and Ki-67 are the two important molecules expressed by the proliferating cells. They are important during the DNA synthesis. We applied the antibodies (using DAKO EPOS system) against these two proteins to a series of 54 cases of colorectal adenocarcinoma of various Dukes' stages in order to observe the degree of expressions and their relationship with Dukes' stage. Our study showed, regardless of the Dukes' stage almost all cases are strongly expressed PCNA. However we failed to demonstrate the expression of Ki-67. We conclude that expression of PCNA is strong in colorectal carcinoma but the degree of expression has no relationship with the Dukes' stage. Expression of Ki-67 is probably best seen if we use fresh tissue rather than formalin fixed paraffin embedded tissue. |
| first_indexed | 2025-11-15T17:49:38Z |
| format | Conference or Workshop Item |
| id | usm-42546 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:49:38Z |
| publishDate | 2001 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-425462020-03-20T04:50:47Z http://eprints.usm.my/42546/ (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system Kamal, Meor Zamari Meor Jaafar, Hasnan Ali, Kholip J, Rosli RB151-214 Theories of disease. Etiology. Pathogenesis Proliferating cell nuclear antigen (PCNA) and Ki-67 are the two important molecules expressed by the proliferating cells. They are important during the DNA synthesis. We applied the antibodies (using DAKO EPOS system) against these two proteins to a series of 54 cases of colorectal adenocarcinoma of various Dukes' stages in order to observe the degree of expressions and their relationship with Dukes' stage. Our study showed, regardless of the Dukes' stage almost all cases are strongly expressed PCNA. However we failed to demonstrate the expression of Ki-67. We conclude that expression of PCNA is strong in colorectal carcinoma but the degree of expression has no relationship with the Dukes' stage. Expression of Ki-67 is probably best seen if we use fresh tissue rather than formalin fixed paraffin embedded tissue. 2001 Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42546/1/GP...Analisa_Eskperimen_PCNA_Dan_Ki-67_Sebagai_Petunjuk_Proliferasi_Sel_Dalam_Kes_Barah_Kolon_Di_HUSM_Dan_Hubungannya_Dengan_Duke%27s_Staging_System...2001...OCR...pdf Kamal, Meor Zamari Meor and Jaafar, Hasnan and Ali, Kholip and J, Rosli (2001) (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system. In: (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system. (Submitted) |
| spellingShingle | RB151-214 Theories of disease. Etiology. Pathogenesis Kamal, Meor Zamari Meor Jaafar, Hasnan Ali, Kholip J, Rosli (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title | (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title_full | (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title_fullStr | (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title_full_unstemmed | (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title_short | (Analysis of the proliferating cell nuclear antigen(PCNA) and Ki-67 as proliferating cell markers) Expressions in colorectal carcinoma at HUSM and its relationship with the duke staging system |
| title_sort | (analysis of the proliferating cell nuclear antigen(pcna) and ki-67 as proliferating cell markers) expressions in colorectal carcinoma at husm and its relationship with the duke staging system |
| topic | RB151-214 Theories of disease. Etiology. Pathogenesis |
| url | http://eprints.usm.my/42546/ http://eprints.usm.my/42546/1/GP...Analisa_Eskperimen_PCNA_Dan_Ki-67_Sebagai_Petunjuk_Proliferasi_Sel_Dalam_Kes_Barah_Kolon_Di_HUSM_Dan_Hubungannya_Dengan_Duke%27s_Staging_System...2001...OCR...pdf |