Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.

Rhizophora apiculata bark is tannin rich waste material from charcoal industry, and was utilized as the solid substrate for the production of tannase in solid substrate fermentation system. Upon improvement of physical and chemical parameters via a simple shake flask system, combination of 0.75 mm a...

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Main Author: Tan , Wee Yee
Format: Thesis
Language:English
Published: 2010
Subjects:
Online Access:http://eprints.usm.my/42536/
http://eprints.usm.my/42536/1/Tan_Wee_Yee24.pdf
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author Tan , Wee Yee
author_facet Tan , Wee Yee
author_sort Tan , Wee Yee
building USM Institutional Repository
collection Online Access
description Rhizophora apiculata bark is tannin rich waste material from charcoal industry, and was utilized as the solid substrate for the production of tannase in solid substrate fermentation system. Upon improvement of physical and chemical parameters via a simple shake flask system, combination of 0.75 mm and 2.00 mm of substrate particles, Czapek-Dox medium (pH7) as moisturizing agent, initial moisture content of 1:1.5 (w/v), incubation temperature at 30°C, mixing at every 24 hours and inoculum size of 1 × 106 spores/ml showed tannase achieved 4.97 U/g of fermented substrate on the 6th day of cultivation time. After supplementation of 5% (w/w) tannic acid and 1% (w/w) of urea, tannase achieved 39.34 U/g of fermented substrate after 6 days of cultivation.
first_indexed 2025-11-15T17:49:36Z
format Thesis
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institution Universiti Sains Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T17:49:36Z
publishDate 2010
recordtype eprints
repository_type Digital Repository
spelling usm-425362019-04-12T05:26:57Z http://eprints.usm.my/42536/ Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation. Tan , Wee Yee QH1 Natural history (General - Including nature conservation, geographical distribution) Rhizophora apiculata bark is tannin rich waste material from charcoal industry, and was utilized as the solid substrate for the production of tannase in solid substrate fermentation system. Upon improvement of physical and chemical parameters via a simple shake flask system, combination of 0.75 mm and 2.00 mm of substrate particles, Czapek-Dox medium (pH7) as moisturizing agent, initial moisture content of 1:1.5 (w/v), incubation temperature at 30°C, mixing at every 24 hours and inoculum size of 1 × 106 spores/ml showed tannase achieved 4.97 U/g of fermented substrate on the 6th day of cultivation time. After supplementation of 5% (w/w) tannic acid and 1% (w/w) of urea, tannase achieved 39.34 U/g of fermented substrate after 6 days of cultivation. 2010-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42536/1/Tan_Wee_Yee24.pdf Tan , Wee Yee (2010) Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation. Masters thesis, Universiti Sains Malaysia.
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Tan , Wee Yee
Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title_full Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title_fullStr Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title_full_unstemmed Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title_short Production Of Tannase By Aspergillus Niger Using Mangrove (Rhizophora Apiculata) Bark Under Solid Substrate Fermentation.
title_sort production of tannase by aspergillus niger using mangrove (rhizophora apiculata) bark under solid substrate fermentation.
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
url http://eprints.usm.my/42536/
http://eprints.usm.my/42536/1/Tan_Wee_Yee24.pdf