Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E
Apolipoprotein E phenotyping studies were performed on 17 samples from blood donors and selected subjects. One unit of plasma of 150- 270 ml were collected from each subject. VLDL (very law density lipoproteins) were separated from plasma by ultracentrifugation. VLOL obtained (10% plasma volume) we...
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| Format: | Conference or Workshop Item |
| Language: | English |
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1999
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| Online Access: | http://eprints.usm.my/42377/ http://eprints.usm.my/42377/1/GP...Pembagunan_Kaedah_Mengfenotip_Dan_Penentukuran_Apolipoproten_E...OCR..pdf |
| _version_ | 1848879543171940352 |
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| author | Rashid, Faridah Abdul |
| author_facet | Rashid, Faridah Abdul |
| author_sort | Rashid, Faridah Abdul |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Apolipoprotein E phenotyping studies were performed on 17 samples from blood donors and selected subjects. One unit of plasma of 150- 270 ml were collected from each subject. VLDL (very law density lipoproteins) were separated from plasma by ultracentrifugation. VLOL obtained (10% plasma
volume) were delipidated to remove lipid components and only proteins remained. Protein electrophoresis by isoelectric focussing technique performed in a pH gradient of 4.0-6.5 resulted in 10 protein bands after staining with Coammassie Brilliant Blue. Bands were road by eye without scanning. The apolipoprotetn E phenotyping results obtained were: E4/3(59%), E3/2(12%), E3/3(18%), E2/2(12%). Two apolipaprotein E phenotypes not seen were E4/4 and E4/2. Two E413 cases were deficient in apolipoprotein A-l: one was ambiguous as to whether it was E4/3 or E4/2. One E3l2 case was deficient in apolipoprotein E, A-1, dan C-11. Three cases of E3/3 were deficient in apolipoprctein A-1 and one of them was also deficient for both A-1 and C-ll. Apolipoprotein E levels was low for one case of E3/2 one
case of E3/3 and one case of E2/2. Continued research in this field has been expanded to include apolipoprotein E genotyping studies and to further accommodate other plasma proteins of clinical interest. |
| first_indexed | 2025-11-15T17:48:54Z |
| format | Conference or Workshop Item |
| id | usm-42377 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:48:54Z |
| publishDate | 1999 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-423772020-03-20T04:35:29Z http://eprints.usm.my/42377/ Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E Rashid, Faridah Abdul RC Internal medicine Apolipoprotein E phenotyping studies were performed on 17 samples from blood donors and selected subjects. One unit of plasma of 150- 270 ml were collected from each subject. VLDL (very law density lipoproteins) were separated from plasma by ultracentrifugation. VLOL obtained (10% plasma volume) were delipidated to remove lipid components and only proteins remained. Protein electrophoresis by isoelectric focussing technique performed in a pH gradient of 4.0-6.5 resulted in 10 protein bands after staining with Coammassie Brilliant Blue. Bands were road by eye without scanning. The apolipoprotetn E phenotyping results obtained were: E4/3(59%), E3/2(12%), E3/3(18%), E2/2(12%). Two apolipaprotein E phenotypes not seen were E4/4 and E4/2. Two E413 cases were deficient in apolipoprotein A-l: one was ambiguous as to whether it was E4/3 or E4/2. One E3l2 case was deficient in apolipoprotein E, A-1, dan C-11. Three cases of E3/3 were deficient in apolipoprctein A-1 and one of them was also deficient for both A-1 and C-ll. Apolipoprotein E levels was low for one case of E3/2 one case of E3/3 and one case of E2/2. Continued research in this field has been expanded to include apolipoprotein E genotyping studies and to further accommodate other plasma proteins of clinical interest. 1999 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.usm.my/42377/1/GP...Pembagunan_Kaedah_Mengfenotip_Dan_Penentukuran_Apolipoproten_E...OCR..pdf Rashid, Faridah Abdul (1999) Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E. In: Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E, 9-10 June 1996, USM Kubang Kerian. (Submitted) |
| spellingShingle | RC Internal medicine Rashid, Faridah Abdul Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title | Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title_full | Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title_fullStr | Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title_full_unstemmed | Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title_short | Pembangunan kaedah mengfenotip dan penentukuran apolipoprotein E |
| title_sort | pembangunan kaedah mengfenotip dan penentukuran apolipoprotein e |
| topic | RC Internal medicine |
| url | http://eprints.usm.my/42377/ http://eprints.usm.my/42377/1/GP...Pembagunan_Kaedah_Mengfenotip_Dan_Penentukuran_Apolipoproten_E...OCR..pdf |