Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis

In order to establish a successful process of enzymatic hydrolysis of palm oil, for the production of palmitic acid and oleic acid, the following objectives have been designated: Screening for potential microorganisms producing a significant amount of extracellular organic solvent tolerant lipase...

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Main Author: Al-Limoun, Muhamad Odeh Atyeh
Format: Thesis
Language:English
Published: 2010
Subjects:
Online Access:http://eprints.usm.my/42309/
http://eprints.usm.my/42309/1/Muhamad_Odeh_Atyeh_Al-Limoun24.pdf
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author Al-Limoun, Muhamad Odeh Atyeh
author_facet Al-Limoun, Muhamad Odeh Atyeh
author_sort Al-Limoun, Muhamad Odeh Atyeh
building USM Institutional Repository
collection Online Access
description In order to establish a successful process of enzymatic hydrolysis of palm oil, for the production of palmitic acid and oleic acid, the following objectives have been designated: Screening for potential microorganisms producing a significant amount of extracellular organic solvent tolerant lipase from indigenous soil samples, optimization of the physicochemical parameters required to achieve maximum lipase production in shake flask system, purification of the enzyme using aqueous two phase system coupled with Sephadex G-75 gel filtration, characterization of the pure enzyme properties and application of the crude lipase preparation in palm oil hydrolysis for the production of palmitic and oleic acids.
first_indexed 2025-11-15T17:48:37Z
format Thesis
id usm-42309
institution Universiti Sains Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T17:48:37Z
publishDate 2010
recordtype eprints
repository_type Digital Repository
spelling usm-423092019-04-12T05:26:58Z http://eprints.usm.my/42309/ Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis Al-Limoun, Muhamad Odeh Atyeh QH1 Natural history (General - Including nature conservation, geographical distribution) In order to establish a successful process of enzymatic hydrolysis of palm oil, for the production of palmitic acid and oleic acid, the following objectives have been designated: Screening for potential microorganisms producing a significant amount of extracellular organic solvent tolerant lipase from indigenous soil samples, optimization of the physicochemical parameters required to achieve maximum lipase production in shake flask system, purification of the enzyme using aqueous two phase system coupled with Sephadex G-75 gel filtration, characterization of the pure enzyme properties and application of the crude lipase preparation in palm oil hydrolysis for the production of palmitic and oleic acids. 2010 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42309/1/Muhamad_Odeh_Atyeh_Al-Limoun24.pdf Al-Limoun, Muhamad Odeh Atyeh (2010) Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis. PhD thesis, Universiti Sains Malaysia.
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Al-Limoun, Muhamad Odeh Atyeh
Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title_full Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title_fullStr Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title_full_unstemmed Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title_short Production, Purification, Characterization And Application Of Organic Solvent Tolerant Lipase From Trichoderma Sp. Bw45 In Palm Oil Hydrolysis
title_sort production, purification, characterization and application of organic solvent tolerant lipase from trichoderma sp. bw45 in palm oil hydrolysis
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
url http://eprints.usm.my/42309/
http://eprints.usm.my/42309/1/Muhamad_Odeh_Atyeh_Al-Limoun24.pdf