Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays

Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB) clots were incu...

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Bibliographic Details
Main Authors: Elnager, Abuzar, Hassan, Rosline, Idris, Zamzuri, Mustafa, Zulkifli, Arfah, Nadiah Wan, Sulaiman, S. A., Siew, Hua Gan, Abdullah, Wan Zaidah
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2015
Subjects:
Online Access:http://eprints.usm.my/39119/
http://eprints.usm.my/39119/1/Fibrinolytic_Activity_and_Dose-Dependent_Effect.pdf
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Summary:Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB) clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00mM). After 3 hours, D-dimer (DD) levels and WB clot weights were measured for each concentration. Thromboelastography (TEG) parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope. Results. Overall, mean DD (