Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies
In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Finlay Ediciones
2010
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| Subjects: | |
| Online Access: | http://eprints.usm.my/39018/ http://eprints.usm.my/39018/1/Evaluation_of_the_potential_of_Mycobacterium_smegmatis_as_vaccine_Candidate_against_tuberculosis_by_in_silico_and_in_vivo_studies.pdf |
| Summary: | In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different
phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent
and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the
regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis
based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M.
tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the
immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of
animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the
potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies |
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