Enteric coating of granules containing the probiotic Lactobacillus acidophilus

In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid m...

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Main Authors: Pyar, Hassan, Peh, Kok Khiang
Format: Article
Language:English
Published: De Gruyter Open 2014
Subjects:
Online Access:http://eprints.usm.my/38976/
http://eprints.usm.my/38976/1/Enteric_coating_of_granules_containing_the_probiotic_Lactobacillus_acidophilus.pdf
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author Pyar, Hassan
Peh, Kok Khiang
author_facet Pyar, Hassan
Peh, Kok Khiang
author_sort Pyar, Hassan
building USM Institutional Repository
collection Online Access
description In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid medium and disintegration time of 1 h in buffer pH 6.8. The amount of Eudragit L30D-55 in the capsules correlated with gastric juice resistance. The best protective qualities against artificial gastric juice were observed when capsules were prepared from granules composed of L. acidophilus, corn starch, lactose monohydrate, polyvinylpyrrolidone and coated with 12.5 % (m/V) of Eudragit L30D-55. Capsule formulation of L. acidophilus in edible broth medium suspension serves as a cheap alternative to the expensive freeze-drying procedure for preparing L. acidophilus. In addition, the enteric coating using Eudragit L30D-55 could protect probiotics from the acidic gastric environment and enhance the bioactivity of probiotics along with replacement of pathogenic microbes in human intestine.
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spelling usm-389762018-02-15T08:01:16Z http://eprints.usm.my/38976/ Enteric coating of granules containing the probiotic Lactobacillus acidophilus Pyar, Hassan Peh, Kok Khiang RS1-441 Pharmacy and materia medica In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid medium and disintegration time of 1 h in buffer pH 6.8. The amount of Eudragit L30D-55 in the capsules correlated with gastric juice resistance. The best protective qualities against artificial gastric juice were observed when capsules were prepared from granules composed of L. acidophilus, corn starch, lactose monohydrate, polyvinylpyrrolidone and coated with 12.5 % (m/V) of Eudragit L30D-55. Capsule formulation of L. acidophilus in edible broth medium suspension serves as a cheap alternative to the expensive freeze-drying procedure for preparing L. acidophilus. In addition, the enteric coating using Eudragit L30D-55 could protect probiotics from the acidic gastric environment and enhance the bioactivity of probiotics along with replacement of pathogenic microbes in human intestine. De Gruyter Open 2014 Article PeerReviewed application/pdf en http://eprints.usm.my/38976/1/Enteric_coating_of_granules_containing_the_probiotic_Lactobacillus_acidophilus.pdf Pyar, Hassan and Peh, Kok Khiang (2014) Enteric coating of granules containing the probiotic Lactobacillus acidophilus. Acta Pharmaceutica, 64 (2). pp. 247-256. ISSN 1330-0075 https://www.ncbi.nlm.nih.gov/pubmed/24914724
spellingShingle RS1-441 Pharmacy and materia medica
Pyar, Hassan
Peh, Kok Khiang
Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title_full Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title_fullStr Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title_full_unstemmed Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title_short Enteric coating of granules containing the probiotic Lactobacillus acidophilus
title_sort enteric coating of granules containing the probiotic lactobacillus acidophilus
topic RS1-441 Pharmacy and materia medica
url http://eprints.usm.my/38976/
http://eprints.usm.my/38976/
http://eprints.usm.my/38976/1/Enteric_coating_of_granules_containing_the_probiotic_Lactobacillus_acidophilus.pdf