Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models

Background Ischemic preconditioning has been shown to improve the outcomes of hypoxic tolerance of the heart, brain, lung, liver, jejunum, skin, and muscle tissues. However, to date, no report of ischemic preconditioning on vascularized bone grafts has been published. Methods Sixteen rabbits were d...

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Main Authors: Halim, Ahmad Sukari, Wan Ahmad Kamal, Wan Syazli Rodzaian, Mohd Noor, Norizal, Abdullah, Shafie
Format: Article
Language:English
Published: The Korean Society of Plastic and Reconstructive Surgeons 2013
Subjects:
Online Access:http://eprints.usm.my/38898/
http://eprints.usm.my/38898/1/Effects_of_Ischemic_Preconditioning_of_Different.pdf
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author Halim, Ahmad Sukari
Wan Ahmad Kamal, Wan Syazli Rodzaian
Mohd Noor, Norizal
Abdullah, Shafie
author_facet Halim, Ahmad Sukari
Wan Ahmad Kamal, Wan Syazli Rodzaian
Mohd Noor, Norizal
Abdullah, Shafie
author_sort Halim, Ahmad Sukari
building USM Institutional Repository
collection Online Access
description Background Ischemic preconditioning has been shown to improve the outcomes of hypoxic tolerance of the heart, brain, lung, liver, jejunum, skin, and muscle tissues. However, to date, no report of ischemic preconditioning on vascularized bone grafts has been published. Methods Sixteen rabbits were divided into four groups with ischemic times of 2, 6, 14, and 18 hours. Half of the rabbits in each group underwent ischemic preconditioning. The osteomyocutaneous flaps consisted of the tibia bone, from which the overlying muscle and skin were raised. The technique of ischemic preconditioning involved applying a vascular clamp to the pedicle for 3 cycles of 10 minutes each. The rabbits then underwent serial plain radiography and computed tomography imaging on the first, second, fourth, and sixth postoperative weeks. Following this, all of the rabbits were sacrificed and histological examinations were performed. Results The results showed that for clinical analysis of the skin flaps and bone grafts, the preconditioned groups showed better survivability. In the plain radiographs, except for two non-preconditioned rabbits with intraoperative ischemic times of 6 hours, all began to show early callus formation at the fourth week. The computed tomography findings showed more callus formation in the preconditioned groups for all of the ischemic times except for the 18-hour group. The histological findings correlated with the radiological findings. There was no statistical significance in the difference between the two groups. Conclusions In conclusion, ischemic preconditioning improved the survivability of skin flaps and increased callus formation during the healing process of vascularized bone grafts.
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spelling usm-388982018-02-14T00:52:26Z http://eprints.usm.my/38898/ Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models Halim, Ahmad Sukari Wan Ahmad Kamal, Wan Syazli Rodzaian Mohd Noor, Norizal Abdullah, Shafie R5-130.5 General works Background Ischemic preconditioning has been shown to improve the outcomes of hypoxic tolerance of the heart, brain, lung, liver, jejunum, skin, and muscle tissues. However, to date, no report of ischemic preconditioning on vascularized bone grafts has been published. Methods Sixteen rabbits were divided into four groups with ischemic times of 2, 6, 14, and 18 hours. Half of the rabbits in each group underwent ischemic preconditioning. The osteomyocutaneous flaps consisted of the tibia bone, from which the overlying muscle and skin were raised. The technique of ischemic preconditioning involved applying a vascular clamp to the pedicle for 3 cycles of 10 minutes each. The rabbits then underwent serial plain radiography and computed tomography imaging on the first, second, fourth, and sixth postoperative weeks. Following this, all of the rabbits were sacrificed and histological examinations were performed. Results The results showed that for clinical analysis of the skin flaps and bone grafts, the preconditioned groups showed better survivability. In the plain radiographs, except for two non-preconditioned rabbits with intraoperative ischemic times of 6 hours, all began to show early callus formation at the fourth week. The computed tomography findings showed more callus formation in the preconditioned groups for all of the ischemic times except for the 18-hour group. The histological findings correlated with the radiological findings. There was no statistical significance in the difference between the two groups. Conclusions In conclusion, ischemic preconditioning improved the survivability of skin flaps and increased callus formation during the healing process of vascularized bone grafts. The Korean Society of Plastic and Reconstructive Surgeons 2013-11 Article PeerReviewed application/pdf en http://eprints.usm.my/38898/1/Effects_of_Ischemic_Preconditioning_of_Different.pdf Halim, Ahmad Sukari and Wan Ahmad Kamal, Wan Syazli Rodzaian and Mohd Noor, Norizal and Abdullah, Shafie (2013) Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models. Archives of Plastic Surgery, 40 (6). pp. 687-696. ISSN 2234-6163 http://dx.doi.org/10.5999/aps.2013.40.6.687
spellingShingle R5-130.5 General works
Halim, Ahmad Sukari
Wan Ahmad Kamal, Wan Syazli Rodzaian
Mohd Noor, Norizal
Abdullah, Shafie
Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title_full Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title_fullStr Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title_full_unstemmed Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title_short Effects of Ischemic Preconditioning of Different Intraoperative Ischemic Times of Vascularized Bone Graft Rabbit Models
title_sort effects of ischemic preconditioning of different intraoperative ischemic times of vascularized bone graft rabbit models
topic R5-130.5 General works
url http://eprints.usm.my/38898/
http://eprints.usm.my/38898/
http://eprints.usm.my/38898/1/Effects_of_Ischemic_Preconditioning_of_Different.pdf