Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2015
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/38683/ http://eprints.usm.my/38683/1/Diagnosis_of_Dengue_Infection_Using_Conventional_and_Biosensor_Based.pdf |
| _version_ | 1848878537779445760 |
|---|---|
| author | Parkash, Om Shueb, Rafidah Hanim |
| author_facet | Parkash, Om Shueb, Rafidah Hanim |
| author_sort | Parkash, Om |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Dengue is an arthropod-borne viral disease caused by four antigenically different
serotypes of dengue virus. This disease is considered as a major public health concern around
the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention
and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from
other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt
and accurate laboratory diagnostic test is urgently required for disease confirmation and patient
triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture,
serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses
the conventional laboratory methods used for the diagnosis of dengue during the acute and
convalescent phase and highlights the advantages and limitations of these routine laboratory tests.
Subsequently, the biosensor based assays developed using various transducers for the detection of
dengue are also reviewed. |
| first_indexed | 2025-11-15T17:32:55Z |
| format | Article |
| id | usm-38683 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:32:55Z |
| publishDate | 2015 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-386832018-02-05T01:57:00Z http://eprints.usm.my/38683/ Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques Parkash, Om Shueb, Rafidah Hanim QR1-502 Microbiology Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed. MDPI 2015 Article PeerReviewed application/pdf en http://eprints.usm.my/38683/1/Diagnosis_of_Dengue_Infection_Using_Conventional_and_Biosensor_Based.pdf Parkash, Om and Shueb, Rafidah Hanim (2015) Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques. Viruses, 7 (10). pp. 5410-5427. ISSN 1999-4915 http://www.mdpi.com/1999-4915/7/10/2877 |
| spellingShingle | QR1-502 Microbiology Parkash, Om Shueb, Rafidah Hanim Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title | Diagnosis of Dengue Infection Using Conventional
and Biosensor Based Techniques |
| title_full | Diagnosis of Dengue Infection Using Conventional
and Biosensor Based Techniques |
| title_fullStr | Diagnosis of Dengue Infection Using Conventional
and Biosensor Based Techniques |
| title_full_unstemmed | Diagnosis of Dengue Infection Using Conventional
and Biosensor Based Techniques |
| title_short | Diagnosis of Dengue Infection Using Conventional
and Biosensor Based Techniques |
| title_sort | diagnosis of dengue infection using conventional
and biosensor based techniques |
| topic | QR1-502 Microbiology |
| url | http://eprints.usm.my/38683/ http://eprints.usm.my/38683/ http://eprints.usm.my/38683/1/Diagnosis_of_Dengue_Infection_Using_Conventional_and_Biosensor_Based.pdf |