CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells
Aedes aegypti is a principal vector responsible for the transmission of dengue viruses (DENV). To date, vector control remains the key option for dengue disease management. To develop new vector control strategies, a more comprehensive understanding of the biological interactions between DENV and Ae...
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MDPI
2014
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| Online Access: | http://eprints.usm.my/38539/ http://eprints.usm.my/38539/1/CPB1_of_Aedes_aegypti_Interacts_with_DENV2_E_Protein.pdf |
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| author | Tham, Hong Wai R. M. T. Balasubramaniam, Vinod Ario Tejo, Bimo Ahmad, Hamdan Syed Hassan, Sharifah |
| author_facet | Tham, Hong Wai R. M. T. Balasubramaniam, Vinod Ario Tejo, Bimo Ahmad, Hamdan Syed Hassan, Sharifah |
| author_sort | Tham, Hong Wai |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Aedes aegypti is a principal vector responsible for the transmission of dengue viruses (DENV). To date, vector control remains the key option for dengue disease management. To develop new vector control strategies, a more comprehensive understanding of the biological interactions between DENV and Ae. aegypti is required. In this study, a cDNA library derived from the midgut of female adult Ae. aegypti was used in yeast two-hybrid (Y2H) screenings against DENV2 envelope (E) protein. Among the many interacting proteins identified, carboxypeptidase B1 (CPB1) was selected, and its biological interaction with E protein in Ae. aegypti primary midgut cells was further validated. Our double immunofluorescent assay showed that CPB1-E interaction occurred in the endoplasmic reticulum (ER) of the Ae. aegypti primary midgut cells. Overexpression of CPB1 in mosquito cells resulted in intracellular DENV2 genomic RNA or virus particle accumulation, with a lower amount of virus release. Therefore, we postulated that in Ae. aegypti midgut cells, CPB1 binds to the E protein deposited on the ER intraluminal membranes and inhibits DENV2 RNA encapsulation, thus inhibiting budding from the ER, and may interfere with immature virus transportation to the trans-Golgi network. |
| first_indexed | 2025-11-15T17:32:17Z |
| format | Article |
| id | usm-38539 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:32:17Z |
| publishDate | 2014 |
| publisher | MDPI |
| recordtype | eprints |
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| spelling | usm-385392018-01-25T06:31:19Z http://eprints.usm.my/38539/ CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells Tham, Hong Wai R. M. T. Balasubramaniam, Vinod Ario Tejo, Bimo Ahmad, Hamdan Syed Hassan, Sharifah QH1 Natural history (General - Including nature conservation, geographical distribution) Aedes aegypti is a principal vector responsible for the transmission of dengue viruses (DENV). To date, vector control remains the key option for dengue disease management. To develop new vector control strategies, a more comprehensive understanding of the biological interactions between DENV and Ae. aegypti is required. In this study, a cDNA library derived from the midgut of female adult Ae. aegypti was used in yeast two-hybrid (Y2H) screenings against DENV2 envelope (E) protein. Among the many interacting proteins identified, carboxypeptidase B1 (CPB1) was selected, and its biological interaction with E protein in Ae. aegypti primary midgut cells was further validated. Our double immunofluorescent assay showed that CPB1-E interaction occurred in the endoplasmic reticulum (ER) of the Ae. aegypti primary midgut cells. Overexpression of CPB1 in mosquito cells resulted in intracellular DENV2 genomic RNA or virus particle accumulation, with a lower amount of virus release. Therefore, we postulated that in Ae. aegypti midgut cells, CPB1 binds to the E protein deposited on the ER intraluminal membranes and inhibits DENV2 RNA encapsulation, thus inhibiting budding from the ER, and may interfere with immature virus transportation to the trans-Golgi network. MDPI 2014-12 Article PeerReviewed application/pdf en http://eprints.usm.my/38539/1/CPB1_of_Aedes_aegypti_Interacts_with_DENV2_E_Protein.pdf Tham, Hong Wai and R. M. T. Balasubramaniam, Vinod and Ario Tejo, Bimo and Ahmad, Hamdan and Syed Hassan, Sharifah (2014) CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells. Viruses, 6 (12). pp. 5028-5046. ISSN 1999-4915 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276941/ |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Tham, Hong Wai R. M. T. Balasubramaniam, Vinod Ario Tejo, Bimo Ahmad, Hamdan Syed Hassan, Sharifah CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title | CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title_full | CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title_fullStr | CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title_full_unstemmed | CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title_short | CPB1 of Aedes aegypti Interacts with DENV2 E Protein and Regulates Intracellular Viral Accumulation and Release from Midgut Cells |
| title_sort | cpb1 of aedes aegypti interacts with denv2 e protein and regulates intracellular viral accumulation and release from midgut cells |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/38539/ http://eprints.usm.my/38539/ http://eprints.usm.my/38539/1/CPB1_of_Aedes_aegypti_Interacts_with_DENV2_E_Protein.pdf |