Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats

Colon cancer remains as a serious health problem around the world despite advances in diagnosis and treatment. Dietary fibers are considered to reduce the risk of colon cancer as they are converted to short chain fatty acids by the presence of anaerobic bacteria in the intestine, but imbalanced d...

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Main Authors: Booupathy, Lokesh Kumar, Venkatachalam, Sathishkumar, Natarajan, Nandakumar, Thamaraiselvan, Rengarajan, Arumugam, Madankumar, Periyasamy, Balasubramanian Maruthaiveeran
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Published: Elsevier 2016
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Online Access:http://eprints.usm.my/38440/
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author Booupathy, Lokesh Kumar
Venkatachalam, Sathishkumar
Natarajan, Nandakumar
Thamaraiselvan, Rengarajan
Arumugam, Madankumar
Periyasamy, Balasubramanian Maruthaiveeran
author_facet Booupathy, Lokesh Kumar
Venkatachalam, Sathishkumar
Natarajan, Nandakumar
Thamaraiselvan, Rengarajan
Arumugam, Madankumar
Periyasamy, Balasubramanian Maruthaiveeran
author_sort Booupathy, Lokesh Kumar
building USM Institutional Repository
collection Online Access
description Colon cancer remains as a serious health problem around the world despite advances in diagnosis and treatment. Dietary fibers are considered to reduce the risk of colon cancer as they are converted to short chain fatty acids by the presence of anaerobic bacteria in the intestine, but imbalanced diet and high fat consumption may promote tumor formation at different sites, including the large bowel via increased bacterial enzymes activity. The present study was conducted to characterize the inhibitory action of myrtenal on bacterial enzymes and aberrant crypt foci (ACF). Experimental colon carcinogenesis induced by 1,2- dimethylhydrazine is histologically, morphologically, and anatomically similar to human colonic epithelial neoplasm. Discrete microscopic mucosal lesions such as ACF and malignant tumors function as important biomarkers in the diagnosis of colon cancer. Methylene blue staining was carried out to visualize the impact of 1,2-dimethylhydrazine and myrtenal. Myrtenal-treated animals showed decreased levels of bacterial enzymes such as b-glucuronidase, b-glucosidase, and mucinase. Characteristic changes in the colon were noticed by inhibiting ACF formation in the colon.
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publishDate 2016
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spelling usm-384402018-01-19T03:39:29Z http://eprints.usm.my/38440/ Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats Booupathy, Lokesh Kumar Venkatachalam, Sathishkumar Natarajan, Nandakumar Thamaraiselvan, Rengarajan Arumugam, Madankumar Periyasamy, Balasubramanian Maruthaiveeran RB Pathology Colon cancer remains as a serious health problem around the world despite advances in diagnosis and treatment. Dietary fibers are considered to reduce the risk of colon cancer as they are converted to short chain fatty acids by the presence of anaerobic bacteria in the intestine, but imbalanced diet and high fat consumption may promote tumor formation at different sites, including the large bowel via increased bacterial enzymes activity. The present study was conducted to characterize the inhibitory action of myrtenal on bacterial enzymes and aberrant crypt foci (ACF). Experimental colon carcinogenesis induced by 1,2- dimethylhydrazine is histologically, morphologically, and anatomically similar to human colonic epithelial neoplasm. Discrete microscopic mucosal lesions such as ACF and malignant tumors function as important biomarkers in the diagnosis of colon cancer. Methylene blue staining was carried out to visualize the impact of 1,2-dimethylhydrazine and myrtenal. Myrtenal-treated animals showed decreased levels of bacterial enzymes such as b-glucuronidase, b-glucosidase, and mucinase. Characteristic changes in the colon were noticed by inhibiting ACF formation in the colon. Elsevier 2016 Article PeerReviewed Booupathy, Lokesh Kumar and Venkatachalam, Sathishkumar and Natarajan, Nandakumar and Thamaraiselvan, Rengarajan and Arumugam, Madankumar and Periyasamy, Balasubramanian Maruthaiveeran (2016) Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats. Journal of Food and Drug Analysis, 24. pp. 206-213. ISSN 1021-9498 http://dx.doi.org/10.1016/j.jfda.2015.07.003
spellingShingle RB Pathology
Booupathy, Lokesh Kumar
Venkatachalam, Sathishkumar
Natarajan, Nandakumar
Thamaraiselvan, Rengarajan
Arumugam, Madankumar
Periyasamy, Balasubramanian Maruthaiveeran
Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title_full Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title_fullStr Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title_full_unstemmed Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title_short Chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in Wistar Rats
title_sort chemopreventive effect of myrtenal on bacterial enzyme activity and the development of 1,2-dimethyl hydrazine-induced aberrant crypt foci in wistar rats
topic RB Pathology
url http://eprints.usm.my/38440/
http://eprints.usm.my/38440/