BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep
The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with Br...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://eprints.usm.my/38408/ http://eprints.usm.my/38408/1/BrdU_Pulse_Labelling_In_Vivo_to_Characterise_Cell_Proliferation_during_Regeneration_and_Repair.pdf |
| _version_ | 1848878460999565312 |
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| author | Yahaya, B. McLachlan, G. Collie, D. D. S. |
| author_facet | Yahaya, B. McLachlan, G. Collie, D. D. S. |
| author_sort | Yahaya, B. |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to
endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied
at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially
disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU
labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing
injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after
injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and
in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when
consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness
of in vivo pulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility
of stem cell approaches in relevant lung disease models. |
| first_indexed | 2025-11-15T17:31:42Z |
| format | Article |
| id | usm-38408 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:31:42Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-384082018-01-17T06:08:54Z http://eprints.usm.my/38408/ BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep Yahaya, B. McLachlan, G. Collie, D. D. S. RK1-715 Dentistry The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness of in vivo pulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility of stem cell approaches in relevant lung disease models. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://eprints.usm.my/38408/1/BrdU_Pulse_Labelling_In_Vivo_to_Characterise_Cell_Proliferation_during_Regeneration_and_Repair.pdf Yahaya, B. and McLachlan, G. and Collie, D. D. S. (2013) BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep. Scientific World Journal, 2013 (871932). pp. 1-5. ISSN 2356-6140 http://dx.doi.org/10.1155/2013/871932 |
| spellingShingle | RK1-715 Dentistry Yahaya, B. McLachlan, G. Collie, D. D. S. BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title | BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title_full | BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title_fullStr | BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title_full_unstemmed | BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title_short | BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep |
| title_sort | brdu pulse labelling in vivo to characterise cell proliferation during regeneration and repair following injury to the airway wall in sheep |
| topic | RK1-715 Dentistry |
| url | http://eprints.usm.my/38408/ http://eprints.usm.my/38408/ http://eprints.usm.my/38408/1/BrdU_Pulse_Labelling_In_Vivo_to_Characterise_Cell_Proliferation_during_Regeneration_and_Repair.pdf |