BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia

Recent studies suggested that Eurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects of Eurycoma longifolia supplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient ost...

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Main Authors: Ramli, Rosmaliza, Khamis, Mohd Fadhli, Shuid, Ahmad Nazrun
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2012
Subjects:
Online Access:http://eprints.usm.my/38407/
http://eprints.usm.my/38407/1/Bone_Micro-CT_Assessments_in_an_Orchidectomised_Rat_Model_Supplemented_with.pdf
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author Ramli, Rosmaliza
Khamis, Mohd Fadhli
Shuid, Ahmad Nazrun
author_facet Ramli, Rosmaliza
Khamis, Mohd Fadhli
Shuid, Ahmad Nazrun
author_sort Ramli, Rosmaliza
building USM Institutional Repository
collection Online Access
description Recent studies suggested that Eurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects of Eurycoma longifolia supplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient osteoporosis model). Forty-eight-aged (10–12 months old) Sprague Dawley rats were divided into six groups of sham-operated (SHAM), orchidectomised control (ORX), orchidectomised + 7mg/rat testosterone enanthate (TEN) and orchidectomised + Eurycoma longifolia 30 mg/kg (EL30), orchidectomised + Eurycoma longifolia 60 mg/kg (EL60), orchidectomised + Eurycoma longifolia 90 mg/kg (EL90). Rats were euthanized following six weeks of treatment. The left femora were used to measure the trabecular bone microarchitecture using micro-CT. Orchidectomy significantly decreased connectivity density, trabecular bone volume, and trabecular number compared to the SHAM group. Testosterone replacement reversed all the orchidectomy-induced changes in the micro-CT parameters. EL at 30 and 60 mg/kg rat worsened the trabecular bone connectivity density and trabecular separation parameters of orchidectomised rats. EL at 90 mg/kg rat preserved the bone volume. High dose of EL (90 mg/kg) may have potential in preserving the bone microarchitecture of orchidectomised rats, but lower doses may further worsen the osteoporotic changes.
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spelling usm-384072018-01-17T05:00:44Z http://eprints.usm.my/38407/ BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia Ramli, Rosmaliza Khamis, Mohd Fadhli Shuid, Ahmad Nazrun RK1-715 Dentistry Recent studies suggested that Eurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects of Eurycoma longifolia supplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient osteoporosis model). Forty-eight-aged (10–12 months old) Sprague Dawley rats were divided into six groups of sham-operated (SHAM), orchidectomised control (ORX), orchidectomised + 7mg/rat testosterone enanthate (TEN) and orchidectomised + Eurycoma longifolia 30 mg/kg (EL30), orchidectomised + Eurycoma longifolia 60 mg/kg (EL60), orchidectomised + Eurycoma longifolia 90 mg/kg (EL90). Rats were euthanized following six weeks of treatment. The left femora were used to measure the trabecular bone microarchitecture using micro-CT. Orchidectomy significantly decreased connectivity density, trabecular bone volume, and trabecular number compared to the SHAM group. Testosterone replacement reversed all the orchidectomy-induced changes in the micro-CT parameters. EL at 30 and 60 mg/kg rat worsened the trabecular bone connectivity density and trabecular separation parameters of orchidectomised rats. EL at 90 mg/kg rat preserved the bone volume. High dose of EL (90 mg/kg) may have potential in preserving the bone microarchitecture of orchidectomised rats, but lower doses may further worsen the osteoporotic changes. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38407/1/Bone_Micro-CT_Assessments_in_an_Orchidectomised_Rat_Model_Supplemented_with.pdf Ramli, Rosmaliza and Khamis, Mohd Fadhli and Shuid, Ahmad Nazrun (2012) BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia. Evidence-Based Complementary and Alternative Medicine, 2012 (501858). pp. 1-9. ISSN 1741-427X https://doi.org/10.1155/2012/501858
spellingShingle RK1-715 Dentistry
Ramli, Rosmaliza
Khamis, Mohd Fadhli
Shuid, Ahmad Nazrun
BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title_full BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title_fullStr BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title_full_unstemmed BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title_short BoneMicro-CT Assessments in an Orchidectomised RatModel Supplemented with Eurycoma longifolia
title_sort bonemicro-ct assessments in an orchidectomised ratmodel supplemented with eurycoma longifolia
topic RK1-715 Dentistry
url http://eprints.usm.my/38407/
http://eprints.usm.my/38407/
http://eprints.usm.my/38407/1/Bone_Micro-CT_Assessments_in_an_Orchidectomised_Rat_Model_Supplemented_with.pdf