Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth
The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus (Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical scavenging, and ferrous ion chelating methods...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://eprints.usm.my/38338/ http://eprints.usm.my/38338/1/Antioxidant_and_Toxicity_Studies_of_50%25_Methanolic_Extract_of_Orthosiphon_stamineus_Benth.pdf |
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| author | Mun, Fei Yam Chung, Pin Lim Lee, Fung Ang Lip, Yee Por Siew, Tung Wong Asmawi, Mohd. Zaini Basir, Rusliza Ahmad, Mariam |
| author_facet | Mun, Fei Yam Chung, Pin Lim Lee, Fung Ang Lip, Yee Por Siew, Tung Wong Asmawi, Mohd. Zaini Basir, Rusliza Ahmad, Mariam |
| author_sort | Mun, Fei Yam |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus
(Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical
scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract. In acute toxicity
study, single dose of MEOS, 5000mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14
days. While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000mg/kg/day for 28
days. From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating,
and antilipid peroxidation activities. There was no mortality detected or any signs of toxicity in acute and subchronic toxicity
studies. Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical
parameters between bothmale and female treated rats in any doses tested.No abnormality of internal organs was observed between
treatment and control groups.Theoral lethal dose determined wasmore than 5000mg/kg and the no-observed-adverse-effect level
(NOAEL) of MEOS for both male and female rats is considered to be 5000mg/kg per day. |
| first_indexed | 2025-11-15T17:31:22Z |
| format | Article |
| id | usm-38338 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:31:22Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-383382018-01-11T02:07:57Z http://eprints.usm.my/38338/ Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth Mun, Fei Yam Chung, Pin Lim Lee, Fung Ang Lip, Yee Por Siew, Tung Wong Asmawi, Mohd. Zaini Basir, Rusliza Ahmad, Mariam RS1-441 Pharmacy and materia medica The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus (Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract. In acute toxicity study, single dose of MEOS, 5000mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14 days. While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000mg/kg/day for 28 days. From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating, and antilipid peroxidation activities. There was no mortality detected or any signs of toxicity in acute and subchronic toxicity studies. Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical parameters between bothmale and female treated rats in any doses tested.No abnormality of internal organs was observed between treatment and control groups.Theoral lethal dose determined wasmore than 5000mg/kg and the no-observed-adverse-effect level (NOAEL) of MEOS for both male and female rats is considered to be 5000mg/kg per day. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://eprints.usm.my/38338/1/Antioxidant_and_Toxicity_Studies_of_50%25_Methanolic_Extract_of_Orthosiphon_stamineus_Benth.pdf Mun, Fei Yam and Chung, Pin Lim and Lee, Fung Ang and Lip, Yee Por and Siew, Tung Wong and Asmawi, Mohd. Zaini and Basir, Rusliza and Ahmad, Mariam (2013) Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth. BioMed Research International, 2013 (351602). pp. 1-10. ISSN 2314-6133 http://dx.doi.org/10.1155/2013/351602 |
| spellingShingle | RS1-441 Pharmacy and materia medica Mun, Fei Yam Chung, Pin Lim Lee, Fung Ang Lip, Yee Por Siew, Tung Wong Asmawi, Mohd. Zaini Basir, Rusliza Ahmad, Mariam Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth |
| title | Antioxidant and Toxicity Studies of 50% Methanolic Extract
of Orthosiphon stamineus Benth |
| title_full | Antioxidant and Toxicity Studies of 50% Methanolic Extract
of Orthosiphon stamineus Benth |
| title_fullStr | Antioxidant and Toxicity Studies of 50% Methanolic Extract
of Orthosiphon stamineus Benth |
| title_full_unstemmed | Antioxidant and Toxicity Studies of 50% Methanolic Extract
of Orthosiphon stamineus Benth |
| title_short | Antioxidant and Toxicity Studies of 50% Methanolic Extract
of Orthosiphon stamineus Benth |
| title_sort | antioxidant and toxicity studies of 50% methanolic extract
of orthosiphon stamineus benth |
| topic | RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/38338/ http://eprints.usm.my/38338/ http://eprints.usm.my/38338/1/Antioxidant_and_Toxicity_Studies_of_50%25_Methanolic_Extract_of_Orthosiphon_stamineus_Benth.pdf |