Short Communication - Current status of Blastocystis in cockroaches

There are few reports on Blastocystis spp. infections in invertebrate hosts namely, cockroaches. Due to their close proximity to humans especially to their dwellings prompted this study as these organisms could possibly play a role in human transmission. A total of 151 cockroaches consisted predo...

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Main Authors: M.T., Farah Haziqah, M.N., Nur Asyiqin, M.K.N., Mohd Khalid, K., Suresh, A., Rajamanikam, P., Chandrawathani, S.N., Mohd Zain
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2017
Subjects:
Online Access:http://eprints.usm.my/38093/
http://eprints.usm.my/38093/1/Current_status_of_Blastocystis_in_cockroaches.pdf
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author M.T., Farah Haziqah
M.N., Nur Asyiqin
M.K.N., Mohd Khalid
K., Suresh
A., Rajamanikam
P., Chandrawathani
S.N., Mohd Zain
author_facet M.T., Farah Haziqah
M.N., Nur Asyiqin
M.K.N., Mohd Khalid
K., Suresh
A., Rajamanikam
P., Chandrawathani
S.N., Mohd Zain
author_sort M.T., Farah Haziqah
building USM Institutional Repository
collection Online Access
description There are few reports on Blastocystis spp. infections in invertebrate hosts namely, cockroaches. Due to their close proximity to humans especially to their dwellings prompted this study as these organisms could possibly play a role in human transmission. A total of 151 cockroaches consisted predominantly of nymph and adult stages were captured from several types of dwellings in the state of Perak and Selangor, Malaysia. Approximately half (40.4%) of the cockroach intestinal contents screened were positive and were found associated to two main factors, host-stage and types of dwellings. The granular and vacuolated forms were the most common cell form found in the in vitro cultures and were morphologically similar to B. hominis. However, the surface coat observed was thick with an electron lucent area observed in the central vacuole. The isolates grew in room temperature but optimal growth was observed at a 24ºC similar to the reptilian Blastocystis with a high number of cells were recovered. Using the DNA barcoding method, two isolates were identified as ST3 (allele 56), one isolate was consider as the new subtype with close relation to allele 114
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spelling usm-380932017-12-27T03:42:03Z http://eprints.usm.my/38093/ Short Communication - Current status of Blastocystis in cockroaches M.T., Farah Haziqah M.N., Nur Asyiqin M.K.N., Mohd Khalid K., Suresh A., Rajamanikam P., Chandrawathani S.N., Mohd Zain QL360-599.82 Invertebrates QR180-189.5 Immunology There are few reports on Blastocystis spp. infections in invertebrate hosts namely, cockroaches. Due to their close proximity to humans especially to their dwellings prompted this study as these organisms could possibly play a role in human transmission. A total of 151 cockroaches consisted predominantly of nymph and adult stages were captured from several types of dwellings in the state of Perak and Selangor, Malaysia. Approximately half (40.4%) of the cockroach intestinal contents screened were positive and were found associated to two main factors, host-stage and types of dwellings. The granular and vacuolated forms were the most common cell form found in the in vitro cultures and were morphologically similar to B. hominis. However, the surface coat observed was thick with an electron lucent area observed in the central vacuole. The isolates grew in room temperature but optimal growth was observed at a 24ºC similar to the reptilian Blastocystis with a high number of cells were recovered. Using the DNA barcoding method, two isolates were identified as ST3 (allele 56), one isolate was consider as the new subtype with close relation to allele 114 Malaysian Society of Parasitology and Tropical Medicine 2017 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/38093/1/Current_status_of_Blastocystis_in_cockroaches.pdf M.T., Farah Haziqah and M.N., Nur Asyiqin and M.K.N., Mohd Khalid and K., Suresh and A., Rajamanikam and P., Chandrawathani and S.N., Mohd Zain (2017) Short Communication - Current status of Blastocystis in cockroaches. Tropical Biomedicine, 34 (3). pp. 741-745. ISSN 01275720 http://msptm.org/journal/
spellingShingle QL360-599.82 Invertebrates
QR180-189.5 Immunology
M.T., Farah Haziqah
M.N., Nur Asyiqin
M.K.N., Mohd Khalid
K., Suresh
A., Rajamanikam
P., Chandrawathani
S.N., Mohd Zain
Short Communication - Current status of Blastocystis in cockroaches
title Short Communication - Current status of Blastocystis in cockroaches
title_full Short Communication - Current status of Blastocystis in cockroaches
title_fullStr Short Communication - Current status of Blastocystis in cockroaches
title_full_unstemmed Short Communication - Current status of Blastocystis in cockroaches
title_short Short Communication - Current status of Blastocystis in cockroaches
title_sort short communication - current status of blastocystis in cockroaches
topic QL360-599.82 Invertebrates
QR180-189.5 Immunology
url http://eprints.usm.my/38093/
http://eprints.usm.my/38093/
http://eprints.usm.my/38093/1/Current_status_of_Blastocystis_in_cockroaches.pdf