Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines
Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract (BPE) of Malaysian stingless bee [Lepidotrigona terminata (L. terminata)] and its synergistic effect with cisplatin (a chemotherapeutic drug) on MCF-7 cancer cell line. Methods: The antioxidant ac...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://eprints.usm.my/37650/ http://eprints.usm.my/37650/1/Bee_pollen_extract_of_Malaysian_stingless_bee_enha.pdf |
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| author | Wan Omar, Wan Adnan Azhar, Nur Asna Fadzilah, Nurdianah Harif Nik Mohamed Kamal, Nik Nur Syazni |
| author_facet | Wan Omar, Wan Adnan Azhar, Nur Asna Fadzilah, Nurdianah Harif Nik Mohamed Kamal, Nik Nur Syazni |
| author_sort | Wan Omar, Wan Adnan |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract (BPE) of Malaysian
stingless bee [Lepidotrigona terminata (L. terminata)] and its synergistic effect with cisplatin (a chemotherapeutic
drug) on MCF-7 cancer cell line.
Methods: The antioxidant activity of BPE from L. terminata was measured by using 1,1-diphenyl-2-picrylhydrazyl
radical (DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined
through using MTT assay on MCF-7 and L929 cell lines. An interaction effect (synergistic, additive and antagonistic)
between BPE and cisplatin was determined by CompuSyn software based on MTT assay data.
Results: The EC50 (50% decrement of DPPH inhibition) of BPE was 0.5 mg/mL. L. terminata BPE exhibited
antiproliferative activity on both cancer and normal cell lines. The IC50 (concentration of drug that was required for 50%
of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/mL whereas in normal cell line L929 was 26 mg/mL. The IC50
for cisplatin on MCF-7 was 20 μmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE
at 15 mg/mL was able to potentiate the inhibitory effect of cisplatin at all different concentrations (2.5–20.0 mg/mL).
The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of
less than 1 reported in the CompuSyn software further proved the synergistic effect between BPE and cisplatin,
suggesting that BPE was working synergistically with cisplatin.
Conclusions: Our study therefore suggested that BPE of Malaysian stingless bee, L. terminata is a potential
chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to
be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs.
The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for
in future studies. |
| first_indexed | 2025-11-15T17:28:21Z |
| format | Article |
| id | usm-37650 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:28:21Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-376502017-11-27T04:43:50Z http://eprints.usm.my/37650/ Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines Wan Omar, Wan Adnan Azhar, Nur Asna Fadzilah, Nurdianah Harif Nik Mohamed Kamal, Nik Nur Syazni RS1-441 Pharmacy and materia medica Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract (BPE) of Malaysian stingless bee [Lepidotrigona terminata (L. terminata)] and its synergistic effect with cisplatin (a chemotherapeutic drug) on MCF-7 cancer cell line. Methods: The antioxidant activity of BPE from L. terminata was measured by using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined through using MTT assay on MCF-7 and L929 cell lines. An interaction effect (synergistic, additive and antagonistic) between BPE and cisplatin was determined by CompuSyn software based on MTT assay data. Results: The EC50 (50% decrement of DPPH inhibition) of BPE was 0.5 mg/mL. L. terminata BPE exhibited antiproliferative activity on both cancer and normal cell lines. The IC50 (concentration of drug that was required for 50% of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/mL whereas in normal cell line L929 was 26 mg/mL. The IC50 for cisplatin on MCF-7 was 20 μmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE at 15 mg/mL was able to potentiate the inhibitory effect of cisplatin at all different concentrations (2.5–20.0 mg/mL). The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of less than 1 reported in the CompuSyn software further proved the synergistic effect between BPE and cisplatin, suggesting that BPE was working synergistically with cisplatin. Conclusions: Our study therefore suggested that BPE of Malaysian stingless bee, L. terminata is a potential chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs. The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for in future studies. Elsevier 2016-03 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/37650/1/Bee_pollen_extract_of_Malaysian_stingless_bee_enha.pdf Wan Omar, Wan Adnan and Azhar, Nur Asna and Fadzilah, Nurdianah Harif and Nik Mohamed Kamal, Nik Nur Syazni (2016) Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines. Asian Pacific Journal of Tropical Biomedicine, 6 (3). pp. 265-269. ISSN 2221-1691 https://www.sciencedirect.com/science/article/pii/S2221169115309436 |
| spellingShingle | RS1-441 Pharmacy and materia medica Wan Omar, Wan Adnan Azhar, Nur Asna Fadzilah, Nurdianah Harif Nik Mohamed Kamal, Nik Nur Syazni Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title | Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title_full | Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title_fullStr | Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title_full_unstemmed | Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title_short | Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| title_sort | bee pollen extract of malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines |
| topic | RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/37650/ http://eprints.usm.my/37650/ http://eprints.usm.my/37650/1/Bee_pollen_extract_of_Malaysian_stingless_bee_enha.pdf |