Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2 Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy
Pseudomonas aeruginosa USM-AR2 culture was proven to produce a glycolipid type biosurfactant, rhamnolipid. Fed-batch cultivation of P. aeruginosa USM-AR2 with different feeding strategies was carried out in bioreactor to increase the rhamnolipid production and productivity. The quantification of...
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| Format: | Article |
| Language: | English |
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IACSIT Press, Singapore
2012
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| Online Access: | http://eprints.usm.my/37293/ http://eprints.usm.my/37293/1/034--ICBLS2012-I30022.pdf |
| _version_ | 1848878153545547776 |
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| author | Md Noh, Nur Asshifa Mohd Salleh, Salwa Abdullah, Amirul Al-Ashraf Mohd Yahya, Ahmad Ramli |
| author_facet | Md Noh, Nur Asshifa Mohd Salleh, Salwa Abdullah, Amirul Al-Ashraf Mohd Yahya, Ahmad Ramli |
| author_sort | Md Noh, Nur Asshifa |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Pseudomonas aeruginosa USM-AR2 culture was proven to produce a glycolipid type
biosurfactant, rhamnolipid. Fed-batch cultivation of P. aeruginosa USM-AR2 with different feeding
strategies was carried out in bioreactor to increase the rhamnolipid production and productivity. The
quantification of cell biomass, rhamnolipid produced and rhamnolipid activity were determined using
absorbance measurement, orcinol assay and surface tension measurement. Batch cultivation in bioreactor
gave a biomass of 9.4 g L-1 and rhamnolipid of 2.35 g L-1 in 72 hours. Fed-batch cultivation with pulse
feeding strategy using diesel as carbon substrate was carried out at different feeding time interval. The best
feeding mode was recorded to be 12 h-feeding interval which resulted in 12.6 g L-1 of biomass and 3.13 g L-1
of rhamnolipid achieved in shorter time, 48 hours cultivation. Higher productivity was achieved by gradually
feeding the carbon source while maintaining the optimal culture conditions, which was 0.065 g L-1 h-1
compared to batch culture, 0.033 g L-1 h-1.The highest biomass and rhamnolipid production, 24.1 g L-1 and
13.4 g L-1, respectively was obtained when multiple substrates were fed (diesel and yeast extract). However
the productivity was lower, 0.054 g L-1 h-1 due to the extension of working time. |
| first_indexed | 2025-11-15T17:26:49Z |
| format | Article |
| id | usm-37293 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:26:49Z |
| publishDate | 2012 |
| publisher | IACSIT Press, Singapore |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-372932017-10-31T09:19:02Z http://eprints.usm.my/37293/ Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2 Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy Md Noh, Nur Asshifa Mohd Salleh, Salwa Abdullah, Amirul Al-Ashraf Mohd Yahya, Ahmad Ramli QH301 Biology Pseudomonas aeruginosa USM-AR2 culture was proven to produce a glycolipid type biosurfactant, rhamnolipid. Fed-batch cultivation of P. aeruginosa USM-AR2 with different feeding strategies was carried out in bioreactor to increase the rhamnolipid production and productivity. The quantification of cell biomass, rhamnolipid produced and rhamnolipid activity were determined using absorbance measurement, orcinol assay and surface tension measurement. Batch cultivation in bioreactor gave a biomass of 9.4 g L-1 and rhamnolipid of 2.35 g L-1 in 72 hours. Fed-batch cultivation with pulse feeding strategy using diesel as carbon substrate was carried out at different feeding time interval. The best feeding mode was recorded to be 12 h-feeding interval which resulted in 12.6 g L-1 of biomass and 3.13 g L-1 of rhamnolipid achieved in shorter time, 48 hours cultivation. Higher productivity was achieved by gradually feeding the carbon source while maintaining the optimal culture conditions, which was 0.065 g L-1 h-1 compared to batch culture, 0.033 g L-1 h-1.The highest biomass and rhamnolipid production, 24.1 g L-1 and 13.4 g L-1, respectively was obtained when multiple substrates were fed (diesel and yeast extract). However the productivity was lower, 0.054 g L-1 h-1 due to the extension of working time. IACSIT Press, Singapore 2012 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/37293/1/034--ICBLS2012-I30022.pdf Md Noh, Nur Asshifa and Mohd Salleh, Salwa and Abdullah, Amirul Al-Ashraf and Mohd Yahya, Ahmad Ramli (2012) Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2 Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy. International Proceedings of Chemical, Biological and Environmental Engineering, 40 (34). pp. 168-174. ISSN 2010-4618 http://www.ipcbee.com/vol40/034--ICBLS2012-I30022.pdf |
| spellingShingle | QH301 Biology Md Noh, Nur Asshifa Mohd Salleh, Salwa Abdullah, Amirul Al-Ashraf Mohd Yahya, Ahmad Ramli Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2 Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title | Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2
Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title_full | Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2
Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title_fullStr | Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2
Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title_full_unstemmed | Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2
Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title_short | Fed-batch Cultivation of Pseudomonas Aeruginosa USM-AR2
Producing Rhamnolipid in Bioreactor through Pulse Feeding Strategy |
| title_sort | fed-batch cultivation of pseudomonas aeruginosa usm-ar2
producing rhamnolipid in bioreactor through pulse feeding strategy |
| topic | QH301 Biology |
| url | http://eprints.usm.my/37293/ http://eprints.usm.my/37293/ http://eprints.usm.my/37293/1/034--ICBLS2012-I30022.pdf |