Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler

Polymerase chain reaction (PCR) is the basis of recombinant and other molecular biological techniques. Availability of cheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Herein we compared the efficacy of a portable thermal cycler ( Palm...

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Main Authors: Ahmed, Siti Aminah, Hong, Leong Cheah, Ravichantar, Nithya, Gopinath, Subash C.B., Thean, Hock Tang
Format: Article
Language:English
Published: Prince of Songkla University 2016
Subjects:
Online Access:http://eprints.usm.my/36935/
http://eprints.usm.my/36935/1/%28Evaluation_of_Palm_PCRTM_G1-12_System%29_38-4-1.pdf
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author Ahmed, Siti Aminah
Hong, Leong Cheah
Ravichantar, Nithya
Gopinath, Subash C.B.
Thean, Hock Tang
author_facet Ahmed, Siti Aminah
Hong, Leong Cheah
Ravichantar, Nithya
Gopinath, Subash C.B.
Thean, Hock Tang
author_sort Ahmed, Siti Aminah
building USM Institutional Repository
collection Online Access
description Polymerase chain reaction (PCR) is the basis of recombinant and other molecular biological techniques. Availability of cheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Herein we compared the efficacy of a portable thermal cycler ( Palm PCRTM G1-12 System) for rapid DNA amplification against the standard Peltier-based thermal cycler using plasmid DNA and genomic DNA in single and multiplex PCR experiments. Our study revealed that the Palm PCRTM G1-12 System could be a portable DNA amplification system to conduct various molecular techniques, especially in places where resources are limited.
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institution Universiti Sains Malaysia
institution_category Local University
language English
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publishDate 2016
publisher Prince of Songkla University
recordtype eprints
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spelling usm-369352017-10-05T00:39:44Z http://eprints.usm.my/36935/ Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler Ahmed, Siti Aminah Hong, Leong Cheah Ravichantar, Nithya Gopinath, Subash C.B. Thean, Hock Tang RK1-715 Dentistry Polymerase chain reaction (PCR) is the basis of recombinant and other molecular biological techniques. Availability of cheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Herein we compared the efficacy of a portable thermal cycler ( Palm PCRTM G1-12 System) for rapid DNA amplification against the standard Peltier-based thermal cycler using plasmid DNA and genomic DNA in single and multiplex PCR experiments. Our study revealed that the Palm PCRTM G1-12 System could be a portable DNA amplification system to conduct various molecular techniques, especially in places where resources are limited. Prince of Songkla University 2016-07 Article PeerReviewed application/pdf en http://eprints.usm.my/36935/1/%28Evaluation_of_Palm_PCRTM_G1-12_System%29_38-4-1.pdf Ahmed, Siti Aminah and Hong, Leong Cheah and Ravichantar, Nithya and Gopinath, Subash C.B. and Thean, Hock Tang (2016) Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler. Songklanakarin Journal of Science and Technology (SJST), 38 (4). pp. 343-348. ISSN 0125-3395 http://rdo.psu.ac.th/sjstweb/journal/38-4/38-4-1.pdf
spellingShingle RK1-715 Dentistry
Ahmed, Siti Aminah
Hong, Leong Cheah
Ravichantar, Nithya
Gopinath, Subash C.B.
Thean, Hock Tang
Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title_full Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title_fullStr Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title_full_unstemmed Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title_short Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler
title_sort evaluation of palm pcrtm g1-12 system: a portable battery-operated pcr thermal cycler
topic RK1-715 Dentistry
url http://eprints.usm.my/36935/
http://eprints.usm.my/36935/
http://eprints.usm.my/36935/1/%28Evaluation_of_Palm_PCRTM_G1-12_System%29_38-4-1.pdf