Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7
Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C. inophyllum) fruit extract against human breast cancer cells MCF-7. Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | http://eprints.usm.my/36912/ |
| _version_ | 1848878044940337152 |
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| author | Perumal, Shanmugapriya Yeng, Chen Kanwar, Jagat Rakesh Sasidharan, Sreenivasan |
| author_facet | Perumal, Shanmugapriya Yeng, Chen Kanwar, Jagat Rakesh Sasidharan, Sreenivasan |
| author_sort | Perumal, Shanmugapriya |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C.
inophyllum) fruit extract against human breast cancer cells MCF-7.
Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was
evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of
treated MCF-7 cells was observed under optical microscope using Giemsa staining.
Results: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was
evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which
demonstrated the inhibition of cell viability with the IC50 values of 19.63 μg/mL and 27.54
μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells
treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract was observed under an
optical microscopy via Giemsa staining, which exhibited prominent histological characteristics
of apoptosis.
Conclusions: This study clearly proved that the proliferation of human breast cancer cell
MCF-7 was inhibited by C. inophyllum fruit extract resulted from the induction of apoptosis in
MCF-7 cells. |
| first_indexed | 2025-11-15T17:25:05Z |
| format | Article |
| id | usm-36912 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T17:25:05Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-369122017-10-04T01:39:17Z http://eprints.usm.my/36912/ Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7 Perumal, Shanmugapriya Yeng, Chen Kanwar, Jagat Rakesh Sasidharan, Sreenivasan R735-854 Medical education. Medical schools. Research Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C. inophyllum) fruit extract against human breast cancer cells MCF-7. Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of treated MCF-7 cells was observed under optical microscope using Giemsa staining. Results: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which demonstrated the inhibition of cell viability with the IC50 values of 19.63 μg/mL and 27.54 μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract was observed under an optical microscopy via Giemsa staining, which exhibited prominent histological characteristics of apoptosis. Conclusions: This study clearly proved that the proliferation of human breast cancer cell MCF-7 was inhibited by C. inophyllum fruit extract resulted from the induction of apoptosis in MCF-7 cells. Elsevier 2016-04 Article PeerReviewed Perumal, Shanmugapriya and Yeng, Chen and Kanwar, Jagat Rakesh and Sasidharan, Sreenivasan (2016) Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7. Asian Pacific Journal of Tropical Disease, 6 (4). pp. 291-297. ISSN 2222-1808 https://doi.org/10.1016/S2222-1808(15)61033-7 |
| spellingShingle | R735-854 Medical education. Medical schools. Research Perumal, Shanmugapriya Yeng, Chen Kanwar, Jagat Rakesh Sasidharan, Sreenivasan Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7 |
| title | Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells MCF-7 |
| title_full | Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells MCF-7 |
| title_fullStr | Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells MCF-7 |
| title_full_unstemmed | Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells MCF-7 |
| title_short | Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells MCF-7 |
| title_sort | effects of calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of
human breast cancer cells mcf-7 |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/36912/ http://eprints.usm.my/36912/ |