Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies
CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubata...
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| Format: | Thesis |
| Language: | English |
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2013
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| Online Access: | http://eprints.usm.my/36415/ http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf |
| _version_ | 1848877903382577152 |
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| author | Lee, Wee Leng |
| author_facet | Lee, Wee Leng |
| author_sort | Lee, Wee Leng |
| building | USM Institutional Repository |
| collection | Online Access |
| description | CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab
dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa
kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan
di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubatan
adalah kekurangan. Objektif penyelidikan ini adalah menghasilkan assei in vitro
berkenaan interaski ubatan-herbal dan mengunakannya untuk menganalisasi interaksi
herbal tempatan. CYP2D6*1 rekombinan telah dihasilkan dan diekpreskan bersama
CYP-reductase dalam bacteria E. coli. CYP2D6* 10 dihasilkan dengan kaedah "sitedirected
mutagenesis" dan diekpreskan sebagai CYP2D6* 1.
CYP2D6 is one of the maJor CYPs enzymes and is responsible for the
metabolism of about 20% of cunently available therapeutic chugs. CYP2D6 is
polymorphic and CYP2D6* 10 is the common allele in the Malaysian population.
There is lack of data on local herbal-drug interaction. The objective of this study was
to generate an in vitro drug-herbs int1eraction assay and use it to analyse local herb
interaction. Recombinant CYP2D6* 1 was generated and co-expressed with CYPreductase
in E. coli. Recombinant CYP2D6*10 was generated using site-directed
mutagenesis and expressed as CYP2D6* 1.
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| first_indexed | 2025-11-15T17:22:50Z |
| format | Thesis |
| id | usm-36415 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:22:50Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-364152019-04-12T05:26:17Z http://eprints.usm.my/36415/ Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies Lee, Wee Leng RM300-666 Drugs and their actions CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubatan adalah kekurangan. Objektif penyelidikan ini adalah menghasilkan assei in vitro berkenaan interaski ubatan-herbal dan mengunakannya untuk menganalisasi interaksi herbal tempatan. CYP2D6*1 rekombinan telah dihasilkan dan diekpreskan bersama CYP-reductase dalam bacteria E. coli. CYP2D6* 10 dihasilkan dengan kaedah "sitedirected mutagenesis" dan diekpreskan sebagai CYP2D6* 1. CYP2D6 is one of the maJor CYPs enzymes and is responsible for the metabolism of about 20% of cunently available therapeutic chugs. CYP2D6 is polymorphic and CYP2D6* 10 is the common allele in the Malaysian population. There is lack of data on local herbal-drug interaction. The objective of this study was to generate an in vitro drug-herbs int1eraction assay and use it to analyse local herb interaction. Recombinant CYP2D6* 1 was generated and co-expressed with CYPreductase in E. coli. Recombinant CYP2D6*10 was generated using site-directed mutagenesis and expressed as CYP2D6* 1. 2013-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf Lee, Wee Leng (2013) Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | RM300-666 Drugs and their actions Lee, Wee Leng Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title | Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title_full | Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title_fullStr | Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title_full_unstemmed | Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title_short | Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies |
| title_sort | cloning and expression of cyp2d6*1 and cyp2d6*10 and its application in vitro drug-herbs interaction studies |
| topic | RM300-666 Drugs and their actions |
| url | http://eprints.usm.my/36415/ http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf |