The effects of acute ethanol administration on ethanol withdrawal-induced anxiety-like syndrome in rats: A biochemical study
Withdrawal from long-term ethanol consumption results in overexcitation of glutamatergic neurotransmission in the amygdala, which induces an anxiety-like syndrome. Most alcoholics that suffer from such symptoms frequently depend on habitual drinking as self-medication to alleviate their symptoms....
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| Format: | Article |
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2016
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| Online Access: | http://eprints.usm.my/35771/ |
| _version_ | 1848877713680498688 |
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| author | Kumar, Jaya Hapidin, Hermizi Get Bee, Yvonne-Tee Ismail, Zalina |
| author_facet | Kumar, Jaya Hapidin, Hermizi Get Bee, Yvonne-Tee Ismail, Zalina |
| author_sort | Kumar, Jaya |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Withdrawal from long-term ethanol consumption results in overexcitation of glutamatergic neurotransmission
in the amygdala, which induces an anxiety-like syndrome. Most alcoholics that suffer from
such symptoms frequently depend on habitual drinking as self-medication to alleviate their symptoms.
Metabotropic glutamate receptor subtype 5 (mGlu5) and protein kinase C (PKC) epsilon have been reported
to mediate acute and chronic effects of ethanol. This study explores the changes in mGlu5 and
PKC epsilon in the amygdala following acute administration of ethanol during ethanol withdrawal (EW)
induced anxiety. Male Wistar rats were fed a modified liquid diet containing low-fat cow milk, sucrose,
and maltodextrin, with a gradual introduction of 2.4%, 4.8% and 7.2% ethanol for 20 days. Six hours into
EW, the rats were intraperitoneally injected with normal saline and ethanol (2.5 g/kg, 20% v/v), and
exposed to open-field and elevated plus maze tests. Then, amygdala tissue was dissected from the rat
brain for Western blot and gene expression studies. EW-induced anxiety was accompanied by a significant
increase in mGlu5, total PKC epsilon, and phosphorylated PKC epsilon protein levels, and also of
mRNA of mGlu5 (GRM5) in the amygdala. Acute administration of ethanol significantly attenuated EWinduced
anxiety as well as an EW-induced increase in GRM5. The acute challenge of ethanol to EW rats
had little effect on the phosphorylated and total protein levels of PKC epsilon in the amygdala. Our results
demonstrate that amygdala PKC epsilon may not be directly involved in the development of anxiety
following EW. |
| first_indexed | 2025-11-15T17:19:49Z |
| format | Article |
| id | usm-35771 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T17:19:49Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-357712017-07-21T07:38:37Z http://eprints.usm.my/35771/ The effects of acute ethanol administration on ethanol withdrawal-induced anxiety-like syndrome in rats: A biochemical study Kumar, Jaya Hapidin, Hermizi Get Bee, Yvonne-Tee Ismail, Zalina RA0421 Public health. Hygiene. Preventive Medicine Withdrawal from long-term ethanol consumption results in overexcitation of glutamatergic neurotransmission in the amygdala, which induces an anxiety-like syndrome. Most alcoholics that suffer from such symptoms frequently depend on habitual drinking as self-medication to alleviate their symptoms. Metabotropic glutamate receptor subtype 5 (mGlu5) and protein kinase C (PKC) epsilon have been reported to mediate acute and chronic effects of ethanol. This study explores the changes in mGlu5 and PKC epsilon in the amygdala following acute administration of ethanol during ethanol withdrawal (EW) induced anxiety. Male Wistar rats were fed a modified liquid diet containing low-fat cow milk, sucrose, and maltodextrin, with a gradual introduction of 2.4%, 4.8% and 7.2% ethanol for 20 days. Six hours into EW, the rats were intraperitoneally injected with normal saline and ethanol (2.5 g/kg, 20% v/v), and exposed to open-field and elevated plus maze tests. Then, amygdala tissue was dissected from the rat brain for Western blot and gene expression studies. EW-induced anxiety was accompanied by a significant increase in mGlu5, total PKC epsilon, and phosphorylated PKC epsilon protein levels, and also of mRNA of mGlu5 (GRM5) in the amygdala. Acute administration of ethanol significantly attenuated EWinduced anxiety as well as an EW-induced increase in GRM5. The acute challenge of ethanol to EW rats had little effect on the phosphorylated and total protein levels of PKC epsilon in the amygdala. Our results demonstrate that amygdala PKC epsilon may not be directly involved in the development of anxiety following EW. 2016 Article PeerReviewed Kumar, Jaya and Hapidin, Hermizi and Get Bee, Yvonne-Tee and Ismail, Zalina (2016) The effects of acute ethanol administration on ethanol withdrawal-induced anxiety-like syndrome in rats: A biochemical study. Alcohol, 50. pp. 9-17. ISSN 0741-8329 http://dx.doi.org/10.1016/j.alcohol.2015.10.001 |
| spellingShingle | RA0421 Public health. Hygiene. Preventive Medicine Kumar, Jaya Hapidin, Hermizi Get Bee, Yvonne-Tee Ismail, Zalina The effects of acute ethanol administration on ethanol withdrawal-induced anxiety-like syndrome in rats: A biochemical study |
| title | The effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
A biochemical study |
| title_full | The effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
A biochemical study |
| title_fullStr | The effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
A biochemical study |
| title_full_unstemmed | The effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
A biochemical study |
| title_short | The effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
A biochemical study |
| title_sort | effects of acute ethanol administration on ethanol
withdrawal-induced anxiety-like syndrome in rats:
a biochemical study |
| topic | RA0421 Public health. Hygiene. Preventive Medicine |
| url | http://eprints.usm.my/35771/ http://eprints.usm.my/35771/ |