Immunomodulatory effects of recombinant BCG expressing MSP-1C of Plasmodium falciparum on LPS- or LPS+IFN-γ-stimulated J774A.1 cells
Macrophage phagocytosis is the first line of defense of the innate immune system against malaria parasite infection. This study evaluated the immunomodulatory effects of BCG and recombinant BCG (rBCG) strains expressing the C-terminus of the merozoite surface protein-1 (MSP-1C) of Plasmodium falci...
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| Format: | Article |
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2014
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| Online Access: | http://eprints.usm.my/35766/ |
| _version_ | 1848877712483024896 |
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| author | Mohamad, Dhaniah Suppian, Rapeah Mohd Nor, Norazmi |
| author_facet | Mohamad, Dhaniah Suppian, Rapeah Mohd Nor, Norazmi |
| author_sort | Mohamad, Dhaniah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Macrophage phagocytosis is the first line of defense of the innate immune system against malaria parasite
infection. This study evaluated the immunomodulatory effects of BCG and recombinant BCG (rBCG) strains expressing
the C-terminus of the merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum on mouse macrophage cell line
J774A.1 in the presence or absence of lipopolysaccharide (LPS) or LPS + IFN-γ. The rBCG strain significantly enhanced
phagocytic activity, production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, nitric oxide (NO), and inducible nitric
oxide synthase (iNOS Mean + SE M (x107 CFU)”) as compared with parental BCG strain, and these activities increased in
the presence of LPS and LPS+IFN-γ. Furthermore, the rBCG strain also significantly reduced the macrophage viability as
well as the rBCG growth suggesting the involvement of macrophage apoptosis. Taken together, these data indicate that
the rBCG strain has an immunomodulatory effect on macrophages, thus strengthen the rational use of rBCG to control
malaria infection. |
| first_indexed | 2025-11-15T17:19:48Z |
| format | Article |
| id | usm-35766 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T17:19:48Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-357662017-07-21T01:46:39Z http://eprints.usm.my/35766/ Immunomodulatory effects of recombinant BCG expressing MSP-1C of Plasmodium falciparum on LPS- or LPS+IFN-γ-stimulated J774A.1 cells Mohamad, Dhaniah Suppian, Rapeah Mohd Nor, Norazmi RA0421 Public health. Hygiene. Preventive Medicine Macrophage phagocytosis is the first line of defense of the innate immune system against malaria parasite infection. This study evaluated the immunomodulatory effects of BCG and recombinant BCG (rBCG) strains expressing the C-terminus of the merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum on mouse macrophage cell line J774A.1 in the presence or absence of lipopolysaccharide (LPS) or LPS + IFN-γ. The rBCG strain significantly enhanced phagocytic activity, production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, nitric oxide (NO), and inducible nitric oxide synthase (iNOS Mean + SE M (x107 CFU)”) as compared with parental BCG strain, and these activities increased in the presence of LPS and LPS+IFN-γ. Furthermore, the rBCG strain also significantly reduced the macrophage viability as well as the rBCG growth suggesting the involvement of macrophage apoptosis. Taken together, these data indicate that the rBCG strain has an immunomodulatory effect on macrophages, thus strengthen the rational use of rBCG to control malaria infection. 2014-07 Article PeerReviewed Mohamad, Dhaniah and Suppian, Rapeah and Mohd Nor, Norazmi (2014) Immunomodulatory effects of recombinant BCG expressing MSP-1C of Plasmodium falciparum on LPS- or LPS+IFN-γ-stimulated J774A.1 cells. Human Vaccines & Immunotherapeutics, 10 (7). pp. 1880-1891. ISSN 2164-5515 http://dx.doi.org/10.4161/hv.28695 |
| spellingShingle | RA0421 Public health. Hygiene. Preventive Medicine Mohamad, Dhaniah Suppian, Rapeah Mohd Nor, Norazmi Immunomodulatory effects of recombinant BCG expressing MSP-1C of Plasmodium falciparum on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title | Immunomodulatory effects of recombinant BCG
expressing MSP-1C of Plasmodium falciparum
on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title_full | Immunomodulatory effects of recombinant BCG
expressing MSP-1C of Plasmodium falciparum
on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title_fullStr | Immunomodulatory effects of recombinant BCG
expressing MSP-1C of Plasmodium falciparum
on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title_full_unstemmed | Immunomodulatory effects of recombinant BCG
expressing MSP-1C of Plasmodium falciparum
on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title_short | Immunomodulatory effects of recombinant BCG
expressing MSP-1C of Plasmodium falciparum
on LPS- or LPS+IFN-γ-stimulated J774A.1 cells |
| title_sort | immunomodulatory effects of recombinant bcg
expressing msp-1c of plasmodium falciparum
on lps- or lps+ifn-γ-stimulated j774a.1 cells |
| topic | RA0421 Public health. Hygiene. Preventive Medicine |
| url | http://eprints.usm.my/35766/ http://eprints.usm.my/35766/ |