Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy

A rapid method for quantitative analysis of free fatty acids in virgin coconut oil (VCO) was determined using Fourier transform infrared (FTIR) spectroscopy. Thirty training samples of VCO were prepared by spiking lauric acid to VCO samples covering a range of 0.1% to 5% of free fatty acids. FTIR sp...

Full description

Bibliographic Details
Main Authors: Marina, A. M., Wan Rosli, W. I., Norhidayah, M.
Format: Article
Language:English
Published: School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2015
Subjects:
Online Access:http://eprints.usm.my/31739/
http://eprints.usm.my/31739/1/Wan_Rosli_WI_MUHA_2.pdf
_version_ 1848876644729618432
author Marina, A. M.
Wan Rosli, W. I.
Norhidayah, M.
author_facet Marina, A. M.
Wan Rosli, W. I.
Norhidayah, M.
author_sort Marina, A. M.
building USM Institutional Repository
collection Online Access
description A rapid method for quantitative analysis of free fatty acids in virgin coconut oil (VCO) was determined using Fourier transform infrared (FTIR) spectroscopy. Thirty training samples of VCO were prepared by spiking lauric acid to VCO samples covering a range of 0.1% to 5% of free fatty acids. FTIR spectra between 1730 and 1690 cm-1 was used for quantitative determination of free fatty acids. A partial least square (PLS) model was calibrated to predict the free fatty acids content of VCO. A linear calibration curve was obtained for the actual value against FTIR predicted value which yields an equation of y = 0.9744x +0.0006 and satisfactory results of root mean square error of prediction (RMSEP) of 0.1264 and correlation coefficient (R2) of 0.9281. Thus, FTIR spectroscopy with PLS regression can serve as important tools for determining the free fatty acids in VCO because it is rapid, accurate and environmental friendly compared to the conventional chemical analyses.
first_indexed 2025-11-15T17:02:50Z
format Article
id usm-31739
institution Universiti Sains Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T17:02:50Z
publishDate 2015
publisher School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia
recordtype eprints
repository_type Digital Repository
spelling usm-317392017-08-28T05:02:45Z http://eprints.usm.my/31739/ Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy Marina, A. M. Wan Rosli, W. I. Norhidayah, M. RA0421 Public health. Hygiene. Preventive Medicine TX341-641 Nutrition. Foods and food supply A rapid method for quantitative analysis of free fatty acids in virgin coconut oil (VCO) was determined using Fourier transform infrared (FTIR) spectroscopy. Thirty training samples of VCO were prepared by spiking lauric acid to VCO samples covering a range of 0.1% to 5% of free fatty acids. FTIR spectra between 1730 and 1690 cm-1 was used for quantitative determination of free fatty acids. A partial least square (PLS) model was calibrated to predict the free fatty acids content of VCO. A linear calibration curve was obtained for the actual value against FTIR predicted value which yields an equation of y = 0.9744x +0.0006 and satisfactory results of root mean square error of prediction (RMSEP) of 0.1264 and correlation coefficient (R2) of 0.9281. Thus, FTIR spectroscopy with PLS regression can serve as important tools for determining the free fatty acids in VCO because it is rapid, accurate and environmental friendly compared to the conventional chemical analyses. School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2015 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/31739/1/Wan_Rosli_WI_MUHA_2.pdf Marina, A. M. and Wan Rosli, W. I. and Norhidayah, M. (2015) Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy. Malaysian Applied Biology, 44 (2). pp. 45-49. ISSN 0126-8643 http://mabjournal.com/
spellingShingle RA0421 Public health. Hygiene. Preventive Medicine
TX341-641 Nutrition. Foods and food supply
Marina, A. M.
Wan Rosli, W. I.
Norhidayah, M.
Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title_full Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title_fullStr Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title_full_unstemmed Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title_short Rapid quantification of free fatty acids in virgin coconut oil by FTIR spectroscopy
title_sort rapid quantification of free fatty acids in virgin coconut oil by ftir spectroscopy
topic RA0421 Public health. Hygiene. Preventive Medicine
TX341-641 Nutrition. Foods and food supply
url http://eprints.usm.my/31739/
http://eprints.usm.my/31739/
http://eprints.usm.my/31739/1/Wan_Rosli_WI_MUHA_2.pdf