Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME)
Introduction: The therapeutic potential of plant-based or herbal medicine has been widely embraced by the public as a prevention and remedy for many illnesses. Mitragyna speciosa is one of the medicinal plant that exhibit the opioid-like effects of analgesia. This study aimed to evaluate the phytoch...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/99315/ http://psasir.upm.edu.my/id/eprint/99315/1/2022121912271114_MJMHS_0643.pdf |
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| author | Zailan, Nur Fatin Zalikha Sarchio, Seri Narti Edayu Hassan, Masriana |
| author_facet | Zailan, Nur Fatin Zalikha Sarchio, Seri Narti Edayu Hassan, Masriana |
| author_sort | Zailan, Nur Fatin Zalikha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: The therapeutic potential of plant-based or herbal medicine has been widely embraced by the public as a prevention and remedy for many illnesses. Mitragyna speciosa is one of the medicinal plant that exhibit the opioid-like effects of analgesia. This study aimed to evaluate the phytochemical content, antioxidant activity, and alpha-amylase enzyme inhibition of Mitragyna speciosa methanolic extract (MSME). Methods: The phytochemical composition of MSME was analyzed for secondary metabolites using UHPLC-TWIMS-QTOF-MS/MS. The total phe- nolic content (TPC), total flavonoid content (TFC), antioxidant scavenging activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) [ABTS] assays) and α-amylase enzyme inhi- bition activities of MSME were analyzed in comparison to positive control Pterostilbene and acarbose, respectively. Results: Analysis of UHPLC-TWIMS-QTOF-MS/MS has characterized the presence of at least five different bioactive compounds, mostly derivatives of flavonoids and polyphenols. A significantly higher level of TFC (347.72±15.97 mg QE/g extract; p = 0.0005), but a significantly lower level of TPC (167.43±13.50 mg GAE/g extract; p = 0.002) was detected in MSME (1 mg/mL) compared to Pterostilbene. MSME presented antioxidant activity which has no significant difference compared to Pterostilbene as determined by DPPH (MSME IC50 = 4.34±1.79 µg/mL; p = 0.727) and ABTS (MSME IC50 = 4.25±1.59 µg/mL; p = 0.311) assays. Moreover, MSME also exhibited anti-diabetic effects through inhibition of α-amylase activity (IC50 = 0.01±7.18 mg/mL) which shows a significant difference (p = 0.009) compared to acarbose. Conclusion: This finding suggests that MSME has bioactive phytochemicals and exhibits po- tential antioxidant and anti-diabetic properties. |
| first_indexed | 2025-11-15T13:27:32Z |
| format | Article |
| id | upm-99315 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:27:32Z |
| publishDate | 2022 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| recordtype | eprints |
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| spelling | upm-993152023-03-09T02:50:49Z http://psasir.upm.edu.my/id/eprint/99315/ Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) Zailan, Nur Fatin Zalikha Sarchio, Seri Narti Edayu Hassan, Masriana Introduction: The therapeutic potential of plant-based or herbal medicine has been widely embraced by the public as a prevention and remedy for many illnesses. Mitragyna speciosa is one of the medicinal plant that exhibit the opioid-like effects of analgesia. This study aimed to evaluate the phytochemical content, antioxidant activity, and alpha-amylase enzyme inhibition of Mitragyna speciosa methanolic extract (MSME). Methods: The phytochemical composition of MSME was analyzed for secondary metabolites using UHPLC-TWIMS-QTOF-MS/MS. The total phe- nolic content (TPC), total flavonoid content (TFC), antioxidant scavenging activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) [ABTS] assays) and α-amylase enzyme inhi- bition activities of MSME were analyzed in comparison to positive control Pterostilbene and acarbose, respectively. Results: Analysis of UHPLC-TWIMS-QTOF-MS/MS has characterized the presence of at least five different bioactive compounds, mostly derivatives of flavonoids and polyphenols. A significantly higher level of TFC (347.72±15.97 mg QE/g extract; p = 0.0005), but a significantly lower level of TPC (167.43±13.50 mg GAE/g extract; p = 0.002) was detected in MSME (1 mg/mL) compared to Pterostilbene. MSME presented antioxidant activity which has no significant difference compared to Pterostilbene as determined by DPPH (MSME IC50 = 4.34±1.79 µg/mL; p = 0.727) and ABTS (MSME IC50 = 4.25±1.59 µg/mL; p = 0.311) assays. Moreover, MSME also exhibited anti-diabetic effects through inhibition of α-amylase activity (IC50 = 0.01±7.18 mg/mL) which shows a significant difference (p = 0.009) compared to acarbose. Conclusion: This finding suggests that MSME has bioactive phytochemicals and exhibits po- tential antioxidant and anti-diabetic properties. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/99315/1/2022121912271114_MJMHS_0643.pdf Zailan, Nur Fatin Zalikha and Sarchio, Seri Narti Edayu and Hassan, Masriana (2022) Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME). Malaysian Journal of Medicine and Health Sciences, 18 (supp.21). pp. 92-99. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2022121912271114_MJMHS_0643.pdf 10.47836/mjmhs18.s21.15 |
| spellingShingle | Zailan, Nur Fatin Zalikha Sarchio, Seri Narti Edayu Hassan, Masriana Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title | Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title_full | Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title_fullStr | Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title_full_unstemmed | Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title_short | Evaluation of phytochemical composition, antioxidant and anti- diabetic activities of Mitragyna speciosa Methanolic Extract (MSME) |
| title_sort | evaluation of phytochemical composition, antioxidant and anti- diabetic activities of mitragyna speciosa methanolic extract (msme) |
| url | http://psasir.upm.edu.my/id/eprint/99315/ http://psasir.upm.edu.my/id/eprint/99315/ http://psasir.upm.edu.my/id/eprint/99315/ http://psasir.upm.edu.my/id/eprint/99315/1/2022121912271114_MJMHS_0643.pdf |