Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis

Introduction: Diabetic kidney disease (DKD) is a major global cause of end-stage-kidney disease. In view of its on- going risk of disease progression, the search for a better biomarkers and treatment led to the discovery of microRNAs which regulate gene expression post-translationally. Recently, we...

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Main Authors:	Zahari Sham, Siti Yazmin, Azwar, Shamin, Wai, Kien Yip, Abdullah, Maha, Thevandran, Kalaiselvam, Osman, Malina, Heng, Fong Seow
Format:	Article
Language:	English
Published:	Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/99297/ http://psasir.upm.edu.my/id/eprint/99297/1/2022121911522406_MJMHS_0499.pdf

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author	Zahari Sham, Siti Yazmin Azwar, Shamin Wai, Kien Yip Abdullah, Maha Thevandran, Kalaiselvam Osman, Malina Heng, Fong Seow
author_facet	Zahari Sham, Siti Yazmin Azwar, Shamin Wai, Kien Yip Abdullah, Maha Thevandran, Kalaiselvam Osman, Malina Heng, Fong Seow
author_sort	Zahari Sham, Siti Yazmin
building	UPM Institutional Repository
collection	Online Access
description	Introduction: Diabetic kidney disease (DKD) is a major global cause of end-stage-kidney disease. In view of its on- going risk of disease progression, the search for a better biomarkers and treatment led to the discovery of microRNAs which regulate gene expression post-translationally. Recently, we reported a trend of upregulation of miR-145-5p in sera of type 2 diabetic patients with macroalbuminuria in a selected Malaysian population, which concurred with previous in vivo and in vitro studies of DKD. In addition, miR-145 has been implicated as a tumour suppressor in various cancers. Methods: In this study, bioinformatics tools were utilized to predict the mRNA targets of miR- 145-5p. Results: A total of 683 and 224 experimentally-validated mRNA targets of miR-145-5p were identified by Tarbase and miRTarbase, respectively. Eighty-six (86) commonly identified targets were submitted to Metascape and Enrichr for enrichment analysis. Bioinformatics analysis and literature search suggested that insulin receptor substrate 1 (IRS1) was the most promising target of miR-145-5p. Its associated Gene Ontology terms and pathways included insulin-like growth factor receptor signalling and Forkhead transcription factors (FOXO), respectively. Based on these analyses, the roles of IRS1 in DKD were proposed. Conclusion: As the kidneys are heterogenous in cell types and the mechanism of miRNA is cell-type-dependent, target prediction of miR-145-5p by bioinformatics analysis is particularly important in DKD, to improve the likelihood of a successful in vitro experimental verification in specific renal cell types. In addition, this study attempts to utilize bioinformatics studies, which is not widely done in DKD, as recently reported.
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format	Article
id	upm-99297
institution	Universiti Putra Malaysia
institution_category	Local University
language	English
last_indexed	2025-11-15T13:27:27Z
publishDate	2022
publisher	Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
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spelling	upm-992972023-03-06T07:34:22Z http://psasir.upm.edu.my/id/eprint/99297/ Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis Zahari Sham, Siti Yazmin Azwar, Shamin Wai, Kien Yip Abdullah, Maha Thevandran, Kalaiselvam Osman, Malina Heng, Fong Seow Introduction: Diabetic kidney disease (DKD) is a major global cause of end-stage-kidney disease. In view of its on- going risk of disease progression, the search for a better biomarkers and treatment led to the discovery of microRNAs which regulate gene expression post-translationally. Recently, we reported a trend of upregulation of miR-145-5p in sera of type 2 diabetic patients with macroalbuminuria in a selected Malaysian population, which concurred with previous in vivo and in vitro studies of DKD. In addition, miR-145 has been implicated as a tumour suppressor in various cancers. Methods: In this study, bioinformatics tools were utilized to predict the mRNA targets of miR- 145-5p. Results: A total of 683 and 224 experimentally-validated mRNA targets of miR-145-5p were identified by Tarbase and miRTarbase, respectively. Eighty-six (86) commonly identified targets were submitted to Metascape and Enrichr for enrichment analysis. Bioinformatics analysis and literature search suggested that insulin receptor substrate 1 (IRS1) was the most promising target of miR-145-5p. Its associated Gene Ontology terms and pathways included insulin-like growth factor receptor signalling and Forkhead transcription factors (FOXO), respectively. Based on these analyses, the roles of IRS1 in DKD were proposed. Conclusion: As the kidneys are heterogenous in cell types and the mechanism of miRNA is cell-type-dependent, target prediction of miR-145-5p by bioinformatics analysis is particularly important in DKD, to improve the likelihood of a successful in vitro experimental verification in specific renal cell types. In addition, this study attempts to utilize bioinformatics studies, which is not widely done in DKD, as recently reported. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/99297/1/2022121911522406_MJMHS_0499.pdf Zahari Sham, Siti Yazmin and Azwar, Shamin and Wai, Kien Yip and Abdullah, Maha and Thevandran, Kalaiselvam and Osman, Malina and Heng, Fong Seow (2022) Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis. Malaysian Journal of Medicine and Health Sciences, 18 (supp.21). pp. 36-43. ISSN 2636-9346 (In Press) 10.47836/mjmhs18.s21.7
spellingShingle	Zahari Sham, Siti Yazmin Azwar, Shamin Wai, Kien Yip Abdullah, Maha Thevandran, Kalaiselvam Osman, Malina Heng, Fong Seow Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title	Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title_full	Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title_fullStr	Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title_full_unstemmed	Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title_short	Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis
title_sort	elucidation of mrna targets of mir-145-5p in diabetic kidney disease using bioinformatics analysis
url	http://psasir.upm.edu.my/id/eprint/99297/ http://psasir.upm.edu.my/id/eprint/99297/ http://psasir.upm.edu.my/id/eprint/99297/1/2022121911522406_MJMHS_0499.pdf

Elucidation of mRNA targets of miR-145-5p in diabetic kidney disease using bioinformatics analysis

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