Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds
Pasteurella multocida has been recognized as the causative agent of various infections in a wide range of mammals and bird species. Pasteurella multocida could lead to significant losses in both poultry and wild birds. In poultry, P. multocida can cause acute septicemic disease with high morbidit...
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| Format: | Thesis |
| Language: | English |
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2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/99199/ http://psasir.upm.edu.my/id/eprint/99199/1/FPV%202020%2016%20IR.pdf |
| _version_ | 1848863067602944000 |
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| author | Ateih, Sabsabi Mohammad |
| author_facet | Ateih, Sabsabi Mohammad |
| author_sort | Ateih, Sabsabi Mohammad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pasteurella multocida has been recognized as the causative agent of various infections
in a wide range of mammals and bird species. Pasteurella multocida could lead to
significant losses in both poultry and wild birds. In poultry, P. multocida can cause acute
septicemic disease with high morbidity and mortality; however, localized chronic
infection is more often reported. Pasteurella multocida can be classified into five
serogroups (A, B, D, E, and F) based on the capsular antigen. The first objective of this
study is to isolate, identify, and serotype P. multocida from poultry farms in the central
region of Peninsular Malaysia. A total of 372 samples, consisting of oral swabs and
internal organs of chickens from 31 poultry farms, and 59 oral swabs from free-flying
birds were collected in the central region of Peninsular Malaysia. P. multocida was
identified using biochemical test and polymerase chain reaction (PCR). Thirty-four
samples from five chicken farms were positive for P. multocida, while none of the freeflying
birds contained P. multocida. All isolates were identified as serotype A, except
for one isolate as serotype F using the PCR method. The second objective is to determine
the antimicrobial susceptibility profile using disc diffusion method among P. multocida
isolates. Test against commonly used antibiotics in poultry showed that these isolates
showed high rate of resistance against erythromycin (100%), moderate rate of resistance
against streptomycin (80%), enrofloxacin (80%), and susceptible to penicillin G (100%),
amoxicillin (100%), gentamicin (97%), florfenicol (97%), and tetracycline (94%). The
third objective is to molecularly characterize P. multocida isolates using pulsed-field gel
electrophoresis (PFGE) and multilocus sequence typing (MLST). Pulsed-field gel
electrophoresis results showed that 31 isolates were typable, with four distinct profiles
evident among the isolates. Multi Locus Sequence Typing typing was conducted on one
isolate per farm and the untypeable PFGE isolates. Seven different sequence types (ST)
were identified, namely ST8, ST200, ST157, ST214, ST354, ST356, and ST357. Of
these, ST354, ST356, and ST357 were identified for the first time. The fourth objective
is to characterize P. multocida isolated from chicken farms in the central region of
Peninsular Malaysia in between year 2000 to 2018. Thirteen isolates of P. multocida from samples submitted to the bacteriology laboratory, Faculty of Veterinary Medicine,
University Putra Malaysia, Malaysia, were characterized in this objective. These isolates
showed several multidrug-resistant properties against antibiotics. Three different
sequence types (ST) were identified among the laboratory isolates. The finding of the
study provided additional epidemiological information on the strains of P. multocida that
cause outbreaks of fowl cholera in the central region of Peninsular Malaysia. The results
of this study contribute to the understanding of fowl cholera status in Malaysia with
relations to the genetic diversity and antimicrobial resistance. |
| first_indexed | 2025-11-15T13:27:02Z |
| format | Thesis |
| id | upm-99199 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:27:02Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-991992023-03-06T07:32:23Z http://psasir.upm.edu.my/id/eprint/99199/ Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds Ateih, Sabsabi Mohammad Pasteurella multocida has been recognized as the causative agent of various infections in a wide range of mammals and bird species. Pasteurella multocida could lead to significant losses in both poultry and wild birds. In poultry, P. multocida can cause acute septicemic disease with high morbidity and mortality; however, localized chronic infection is more often reported. Pasteurella multocida can be classified into five serogroups (A, B, D, E, and F) based on the capsular antigen. The first objective of this study is to isolate, identify, and serotype P. multocida from poultry farms in the central region of Peninsular Malaysia. A total of 372 samples, consisting of oral swabs and internal organs of chickens from 31 poultry farms, and 59 oral swabs from free-flying birds were collected in the central region of Peninsular Malaysia. P. multocida was identified using biochemical test and polymerase chain reaction (PCR). Thirty-four samples from five chicken farms were positive for P. multocida, while none of the freeflying birds contained P. multocida. All isolates were identified as serotype A, except for one isolate as serotype F using the PCR method. The second objective is to determine the antimicrobial susceptibility profile using disc diffusion method among P. multocida isolates. Test against commonly used antibiotics in poultry showed that these isolates showed high rate of resistance against erythromycin (100%), moderate rate of resistance against streptomycin (80%), enrofloxacin (80%), and susceptible to penicillin G (100%), amoxicillin (100%), gentamicin (97%), florfenicol (97%), and tetracycline (94%). The third objective is to molecularly characterize P. multocida isolates using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pulsed-field gel electrophoresis results showed that 31 isolates were typable, with four distinct profiles evident among the isolates. Multi Locus Sequence Typing typing was conducted on one isolate per farm and the untypeable PFGE isolates. Seven different sequence types (ST) were identified, namely ST8, ST200, ST157, ST214, ST354, ST356, and ST357. Of these, ST354, ST356, and ST357 were identified for the first time. The fourth objective is to characterize P. multocida isolated from chicken farms in the central region of Peninsular Malaysia in between year 2000 to 2018. Thirteen isolates of P. multocida from samples submitted to the bacteriology laboratory, Faculty of Veterinary Medicine, University Putra Malaysia, Malaysia, were characterized in this objective. These isolates showed several multidrug-resistant properties against antibiotics. Three different sequence types (ST) were identified among the laboratory isolates. The finding of the study provided additional epidemiological information on the strains of P. multocida that cause outbreaks of fowl cholera in the central region of Peninsular Malaysia. The results of this study contribute to the understanding of fowl cholera status in Malaysia with relations to the genetic diversity and antimicrobial resistance. 2019-11 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/99199/1/FPV%202020%2016%20IR.pdf Ateih, Sabsabi Mohammad (2019) Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds. Masters thesis, Universiti Putra Malaysia. Pasteurella multocida - Case studies Poultry - Diseases |
| spellingShingle | Pasteurella multocida - Case studies Poultry - Diseases Ateih, Sabsabi Mohammad Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title | Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title_full | Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title_fullStr | Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title_full_unstemmed | Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title_short | Molecular characterization and antibiotic susceptibility of Pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| title_sort | molecular characterization and antibiotic susceptibility of pasteurella multocida isolated from commercial poultry farm and free-flying birds |
| topic | Pasteurella multocida - Case studies Poultry - Diseases |
| url | http://psasir.upm.edu.my/id/eprint/99199/ http://psasir.upm.edu.my/id/eprint/99199/1/FPV%202020%2016%20IR.pdf |